不同麻醉方法对大鼠重症胰腺炎肺微循环的影响  

Effects of Different Anesthetic Methods on Lung Microcirculation in Severe Pancreatitis Rats

作　　者：李佳佳 王燕[1] 齐文杰[1] LI Jia-jia;WANGYan;QI Wen-jie(Department of Infection Diseases,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院感染内科,北京100050

出　　处：《微循环学杂志》2024年第2期7-11,共5页Chinese Journal of Microcirculation

基　　金：首都卫生发展科研专项项目基金(2016-3-2023)。

摘　　要：目的:对比水合氯醛注射麻醉与异氟烷吸入麻醉方法对重症急性胰腺炎急性肺损伤(SAP-ALI)大鼠肺组织损伤严重程度及微循环的影响,指导SAP-ALI动物模型的建立。方法:30只雄性Wistar大鼠,随机分为3组,分别应用水合氯醛注射麻醉(注射组,n=12)和异氟烷吸入麻醉(吸入组,n=12)的方法建立大鼠SAP-ALI模型,余6只作为对照(对照组),观察大鼠24h死亡率和腹腔内组织大体病理改变,应用ELISA检测血淀粉酶的浓度,应用Moor血流血氧检测仪及激光共聚焦血流血氧检测仪检测大鼠活体肺组织血流量变化,观察肺组织及胰腺组织病理变化,比较组间差异。结果:两种麻醉方法均能成功建立大鼠SAP-ALI模型,但与吸入组相比,注射组大鼠死亡率更高,肺微循环血流量更低(P<0.05),肺组织病理表现更重(P<0.05)。结论:应用异氟烷吸入麻醉建立大鼠SAP-ALI造模可提高实验结果的准确性。Objective:To observe and compare effects of chloral hydrate injection anesthesia and isoflurane inhalation anesthesia on the severity of lung tissue and microcirculation in rats with severe acute pancreatitis-associated acute lung injury(SAP-ALI),and to guide the establishment of the SAP-ALI animal model.Method:Thirty male Wistar rats were randomly divided into three groups.Rat models of SAP-ALI were established using chloral hydrate injection(injection group,n=12)and isoflurane inhalation anesthesia inhalation group(inhalation group,n=12),respectively.The remaining 6 rats were used as controls(control group).The 24-hour mortality rate and gross pathological changes in intraperitoneal tissues were observed.The concentration of serum amylase was detected by enzyme-linked immunosorbent assay(ELISA).The changes in pulmonary blood flow in rats were detected using Moor blood flow and oxygen monitor and laser confocal blood flow and oxygen monitor.Pathological changes and differences in lung and pancreatic tissues were observed.Results:Both anesthesia methods successfully established rat models of SAP-ALI.However,compared with the inhalation group,the rats in the injection group had higher mortality rates,lower pulmonary microvascular blood flow(P<0.05),and more severe pathological manifestations in the lung tissue(P<0.05).Conclusion:It is recommended to use isoflurane inhalation anesthesia when establishing rat models of SAP-ALI to improve the accuracy of experimental results.

关 键 词：急性胰腺炎肺损伤 麻醉方法 肺微循环 大鼠 

分 类 号：R657.5[医药卫生—外科学]