慢性心力衰竭患者血清miR-122和miR-558水平表达与心功能及心率变异性的相关性研究  

Correlation between Serum miR-122 and miR-558 Levels and Cardiac Function and Heart Rate Variability in Patients with Chronic Heart Failure

作　　者：林艳 但汉良 余扬 高鹏 黄继良 白杨[2] LIN Yan;DAN Hanliang;YU Yang;GAO Peng;HUANG Jiliang;BAI Yang(Department of Cardiovascular Medicine,Wuhan Dongxihu District People’s Hospital,Wuhan 430040,China;Department of Cardiovascular Medicine,Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]武汉市东西湖区人民医院心血管内科,武汉430040 [2]华中科技大学同济医学院附属同济医院心血管内科,武汉430030

出　　处：《现代检验医学杂志》2024年第3期37-41,114,共6页Journal of Modern Laboratory Medicine

摘　　要：目的探讨慢性心力衰竭(chronic heart failure,CHF)患者血清微小RNA-122(miR-122)和微小RNA-558(miR-558)水平表达与心功能及心率变异性的关系。方法收集2019年1月~2022年1月武汉市东西湖区人民医院收治的100例慢性心力衰竭患者作为研究组,同期在该院进行体检的健康者100例作为对照组。采用实时荧光定量PCR法检测两组血清miR-122和miR-558表达水平;比较两组心率变异性指标及心功能指标;采用Pearson法分析慢性心力衰竭患者血清miR-122,miR-558表达水平与心率变异性指标、心功能指标的相关性;用Logistic回归分析影响慢性心力衰竭的危险因素。结果与对照组相比,慢性心力衰竭患者血清miR-122(0.86±0.19 vs 1.07±0.16)及心率变异性指标、左室射血分数(LVEF)均明显降低(t=3.844~15.448),miR-558(1.28±0.27 vs 1.02±0.12)表达水平及左室舒张末期容积指数(LVEDVI)、左室收缩末期容积指数(LVESVI)均明显升高(t=6.794~9.358),差异具有统计学意义(均P<0.05);不同心功能分级I,II级组患者血清miR-122(1.19±0.23,1.04±0.21)表达水平显著高于III,IV级组(0.68±0.16,0.53±0.15),miR-558(0.93±0.24,1.21±0.26)表达水平显著低于III,IV级组(1.42±0.27,1.64±0.29),差异具有统计学意义(qmiR-122=3.751~14.568,qmiR-558=3.929~11.194,均P<0.05)。慢性心力衰竭患者血清miR-122与心率变异性指标、LVEF均呈正相关性(r=0.337~0.573),与LVEDVI,LVESVI呈负相关性(r=-0.385,-0.323),血清miR-558与心率变异性指标、LVEF均呈负相关性(r=-0.646~-0.246),与LVEDVI,LVESVI呈正相关性(r=0.528,0.547);血清miR-122[OR(95%CI)=0.528(0.328~0.850)],miR-558[OR(95%CI)=2.845(1.364~5.933)]是慢性心力衰竭的影响因素(P<0.05)。结论慢性心力衰竭患者血清miR-122,miR-558表达水平与心功能、心率变异性密切相关,且血清miR-122和miR-558是慢性心力衰竭的影响因素。Objective To explore the relationship between serum microRNA(miR)-122 and microRNA(miR)-558 levels and cardiac function and heart rate variability in patients with chronic heart failure(CHF).Methods A total of 100 patients with chronic heart failure who were hospitalized in Wuhan Dongxihu District Poeple’s Hospital from January 2019 to January 2022 were regarded as the study group,and 100 healthy individuals who underwent physical examinations in the hospital during the same period served as the control group.The real-time fluorescence quantitative PCR method was applied to detect the expression levels of serum miR-122 and miR-558 in two groups.The heart rate variability and cardiac function indicators of the two groups were compared.Pearson method was applied to analyze the correlation between serum miR-122 and miR-558 expression levels,and heart rate variability and cardiac function indicators in patients with chronic heart failure.Logistic regression was applied to analyze the risk factors affecting chronic heart failure.Results Compared with the control group,the serum miR-122(0.86±0.19 vs 1.07±0.16),heart rate variability indicators,and left ventricular ejection fraction(LVEF)of patients with chronic heart failure were decreased(t=3.844~15.448),and the expression level of miR-558(1.28±0.27 vs 1.02±0.12)and left ventricular end diastolic volume index(LVEDVI),left ventricular end systolic volume index(LVESVI)were increased(t=6.794~9.358),and the differences were statistically significant(all P<0.05).The expression level of serum miR-122(1.19±0.23,1.04±0.21)in patients with functional grades I to II was higher than that in patients with grades III to IV(0.68±0.16,0.53±0.15),while the expression level of miR-558(0.93±0.24,1.21±0.26)was lower than that in patients with grade III-IV(1.42±0.27,1.64±0.29),with significant differences(qmiR-122=3.751~14.568,qmiR-558=3.929~11.194,all P<0.05).The serum miR-122 level in patients with chronic heart failure was positively correlated with heart rate variabilit

关 键 词：慢性心力衰竭 微小RNA-122 微小RNA-558 心功能 心率变异性 

分 类 号：R541.6[医药卫生—心血管疾病] R392.11[医药卫生—内科学]