高血压并发冠状动脉粥样硬化性心脏病患者血清miR-27a-3p和HOXC6水平表达与冠状动脉病变的相关性研究  

Correlation between Serum miR-27a-3p and HOXC6 Levels and Coronary Artery Disease in Patients with Hypertension Complicated with Coronary Atherosclerotic Heart Disease

作　　者：孙实 李想 SUN Shi;LI Xiang(Department of Cardiology,Beidahuang Group General Hospital,Harbin 150088,China;Department of Pathology,Beidahuang Group General Hospital,Harbin 150088,China)

机构地区：[1]北大荒集团总医院心内科,哈尔滨150088 [2]北大荒集团总医院病理科,哈尔滨150088

出　　处：《现代检验医学杂志》2024年第3期67-71,182,共6页Journal of Modern Laboratory Medicine

摘　　要：目的探讨高血压并发冠状动脉粥样硬化性心脏病(冠心病)患者血清微小核糖核酸-27a-3p(miR-27a-3p)和同源异型盒C6(homeobox C6,HOXC6)水平表达与冠状动脉病变的相关性,为高血压并发冠心病的防治提供一定参考。方法选取北大荒集团总医院2021年6月~2023年6月接诊的246例高血压患者为研究对象,其中129例高血压并发冠心病患者作为并发组,117例单纯高血压患者作为未并发组。采用实时荧光定量PCR(quantitative real timePCR,qRT-PCR)检测血清miR-27a-3p和HOXC6表达水平;运用Spearman法分析高血压并发冠心病患者血清miR-27a-3p和HOXC6水平与冠状动脉病变的相关性;多因素Logistic回归分析高血压并发冠心病的影响因素;受试者工作特征(receiver operating characteristic,ROC)曲线分析血清miR-27a-3p,HOXC6水平对高血压并发冠心病的预测价值。结果并发组高血压病程(6.69±1.35年)、总胆固醇(total cholesterol,TC)(5.96±0.86mmol/L)、低密度脂蛋白-胆固醇(low-density lipoprotein cholesterol,LDL-C)(3.36±0.51mmol/L)均显著高于未并发组(5.87±1.32年,5.42±0.67mmol/L,2.98±0.48mmol/L),并发组血清miR-27a-3p(1.35±0.34),HOXC6(1.29±0.33)水平均显著高于未并发组(1.06±0.23,1.03±0.25),差异具有统计学意义(t=4.808,5.454,6.001,7.755,6.911,均P<0.05);轻度组、中度组、重度组患者血清miR-27a-3p(0.54±0.13,1.19±0.31,2.18±0.55)和HOXC6(0.47±0.12,1.12±0.31,2.13±0.52)表达水平逐渐升高,差异具有统计学意义(F=191.026,191.026,均P<0.05)。高血压并发冠心病患者血清miR-27a-3p,HOXC6与SYNTAX评分具呈正相关性(r=0.563,0.517,均P<0.05);血清miR-27a-3p[OR(95%CI:1.627(1.177~2.248)]、HOXC6[OR(95%CI):1.715(1.069~2.750)]是影响高血压并发冠心病的独立危险因素(P<0.05);血清miR-27a-3p和HOXC6联合预测高血压并发冠心病的AUC为0.896(95%CI:0.851~0.931),优于各自单独诊断(Z=2.133,2.815,均P<0.05)。结论高血压并发冠心病患者血清miR-27a-3p,HOXC6水平�Objective To investigate the correlation between serum microRNA(miR)-27a-3p and homeobox C6(HOXC6)levels expression and coronary artery disease in patients with hypertension complicated by coronary atherosclerotic heart disease(CHD),and provide some references for the prevention and treatment of hypertension complicated with coronary heart disease.Methods A total of 246 hypertensive patients accepted by Beidahuang Group General Hospital from June 2021 to June 2023 were collected as the study subjects,with 129 hypertensive patients complicated with coronary heart disease as the concurrent group and 117 simple hypertensive patients as the non concurrent group.Quantitative real time fluorescent PCR(qRT-PCR)was applied to detect the expression levels of miR-27a-3p and HOXC6 in serum.Spearman method was applied to analyze the correlation between serum miR-27a-3p and HOXC6 levels and coronary artery disease in hypertensive patients with coronary heart disease.Multivariate logistic regression was applied to analyze the influencing factors of hypertension complicated with coronary heart disease.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of serum miR-27a-3p and HOXC6 levels for hypertension complicated with coronary heart disease.Results The course of hypertension(6.69±1.35年),total cholesterol(TC)(5.96±0.86mmol/L)and low-density lipoprotein cholesterol(LDL-C)(3.36±0.51mmol/L)in the concurrent group were higher than those in the non concurrent group(5.87±1.32年,5.42±0.67mmol/L,2.98±0.48mmol/L),the serum levels of miR-27a-3p(1.35±0.34)and HOXC6(1.29±0.33)in the concurrent group were higher than those in the non concurrent group(1.06±0.23,1.03±0.25),and the differences were statistically significant(t=4.808,5.454,6.001,7.755,6.911,all P<0.05).The expression levels of miR-27a-3p(0.54±0.13,1.19±0.31,2.18±0.55)and HOXC6(0.47±0.12,1.12±0.31,2.13±0.52)in mild,moderate and severe groups were gradually increased,and the differences were significant(F=191.026,191.026,al

关 键 词：微小核糖核酸-27a-3p 同源异型盒C6 高血压 冠状动脉粥样硬化性心脏病 冠状动脉病变 

分 类 号：R541.3[医药卫生—心血管疾病] R392.11[医药卫生—内科学]