姜黄素固体脂质纳米粒制备工艺的优化和评价  

Optimization and Evaluation of Preparation Technology of Curcumin Solid Lipid Nanoparticles

作　　者：岳重群 朱月 陈滕 杨菁 陈智勇 汪祖华 Yue Zhongqun;Zhu Yue;Chen Teng;Yang Jing;Chen Zhiyong;Wang Zuhua(College of Pharmaceutical Sciences,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Nano-drug Technology Research Center of Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵州中医药大学纳米药物技术研究中心,贵州贵阳550025

出　　处：《山东化工》2024年第8期32-38,共7页Shandong Chemical Industry

摘　　要：目的:优化姜黄素固体脂质纳米粒制备工艺,并对其理化性质进行考察。方法:通过考察制备方法及单因素试验确定其最佳制备条件;采用透射电子显微镜及粒径仪器观察姜黄素固体脂质纳米粒的微观形态;采用紫外分光光度法测定姜黄素的药物包封率及载药量。结果:最优制备条件为:V_(有机相)∶V_(水相)=1∶20,m_(单硬酯酸甘油酯)∶m _(PEG单硬脂酸酯)∶m_(辛酸癸酸单硬酯酸酯)=13∶2∶4,姜黄素含量占脂质质量含量为3.5%,超声时间为7 min,搅拌时间为10 min,水浴温度为70℃,泊洛沙姆188质量分数为0.1%;通过最优条件制备得到的产品为黄色透明液体,在透射电镜下呈圆形或圆球形状,平均粒径为(140.4±0.97)nm,PDI为0.237,电位为(-9.29±1.04)mV;测定其包封率和载药量分别为92.66%±0.17%,3.17%±0.47%。结论:本研究所筛选的姜黄素固体脂质纳米粒制备方法简单,理化性质稳定,可为姜黄素纳米制剂的开发和应用奠定实验基础。Objective:To optimize the preparation technology of curcumin solid lipid nanoparticles and investigate its physicochemical properties.Methods:The optimum preparation conditions were established through an investigation of the preparation method and single factor test.The microscopic morphology of curcumin solid lipid nanoparticles was observed using a transmission electron microscope and particle size instrument.The drug entrapment efficiency and drug loading of curcumin were determined using ultraviolet spectrophotometry.Results:The optimum preparation conditions are as follows:V(organic phase)∶V(aqueous phase)=1∶2;m(glycerol monostearate)∶m(PEG monostearate)∶m(caprylic decanoate monostearate)=13∶2∶4,curcumin content accounts for 3.5%of lipid content,ultrasonic time is 7 min,stirring time is 10 min,water bath temperature is 70℃,and the concentration of poloxamer 188 is 0.1%.under the optimal conditions,the yellow transparent liquid was round or spherical under transmission electron microscope.The average particle size was(140.4±0.97)nm,the PDI was 0.237,and the potential was(-9.29±1.04)mV.The encapsulation rate and drug loading were 92.66%±0.17%and 92.49%±0.02%,respectively.Conclusions:The preparation method of curcumin solid lipid nanoparticles screened in this study is simple and has stable physical and chemical properties,which can lay an experimental foundation for the development and application of curcumin nanopreparations.

关 键 词：固体脂质纳米粒 姜黄素 溶剂乳化超声法 单因素实验 

分 类 号：TQ461[化学工程—制药化工] R943[医药卫生—药剂学]