固定化芽孢杆菌的制备及苯酚降解性能研究  

Study on Preparation of Immobilized Bacillus sp.and Degradation Performance of Phenol

作　　者：滕木兰 刘羽[1] 范飞飞 余强 赵悦 杨平超 王梦雨 Teng Mulan;Liu Yu;Fan Feifei;Yu Qiang;Zhao Yue;Yang Pingchao;Wang Mengyu(School of Petroleum Engineering and Environmental Engineering,Yan’an University,Yanan 716000,China)

机构地区：[1]延安大学石油工程与环境工程学院,陕西延安716000

出　　处：《山东化工》2024年第8期49-52,共4页Shandong Chemical Industry

基　　金：陕西省大学生创新创业训练计划项目(S202110719065);延安大学博士启动项目(YDBK08682019-70)。

摘　　要：酚类物质被广泛应用于石油化工、焦化、塑料等领域,含酚废水易对生态环境造成潜在风险。本研究采用海藻酸钠(SA)及聚乙烯醇(PVA)对芽孢杆菌(Bacillus sp.)进行固定化包埋,考察固定化菌的机械性能及对苯酚的降解效果。研究结果表明,普通包埋法添加3%SA+8%PVA和5%SA+4%PVA的固定化菌球降解性能较好,300 mg/L苯酚19 h的去除率达到97.27%和98.54%,菌球机械强度较弱,可重复利用3次。反复冻融法制备的固定化菌块的机械强度明显增强,可循环利用30次,同时降解速度逐渐加快,在第30次循环利用中6 h内可以将300 mg/L的苯酚全部降解,本研究可为固定化微生物的制备技术和酚类物质降解提供理论支撑。Phenolic substances are widely used in petrochemical,coking,plastics and other fields,and phenolic wastewater is easy to cause potential risks to the ecological environment.In this study,sodium alginate(SA)and polyvinyl alcohol(PVA)were used to immobilize Bacillus sp.,and the mechanical properties and phenol degradation effect of the immobilized bacteria were investigated.The results showed that the immobilized microspheres with the addition of 3%SA+8%PVA and 5%SA+4%PVA by ordinary embedding method had better degradation performance,the degradation rates of 300 mg/L phenol reached 97.27%and 98.54%in 19 h,the mechanical strength of microspheres was weak,and the microspheres could be reused for 3 times.The mechanical strength of the immobilized bacteria prepared by repeated freeze-thaw method was significantly enhanced,and it could be recycled for 30 times,while the degradation rate was gradually accelerated,the 300 mg/L phenol can be completely degraded within 6 h of the 30 th recycling.This study could provide theoretical support for the preparation technology of immobilized microorganisms and the degradation of phenolic substances.

关 键 词：固定化 芽孢杆菌 苯酚 包埋法 反复冻融法 

分 类 号：X503[环境科学与工程—环境工程]