二硫化钼量子点的制备及其在纳米载药体系中的应用  

Preparation of Molybdenum Disulfide Quantum Dots and Their Application in Nanoparticle Drug Delivery System

作　　者：高培轩 刘莉 张乃强 韩馨 董建 Gao Peixuan;Liu Li;Zhang Naiqiang;Han Xin;Dong Jian(Shandong First Medical University&Shandong Academy of Medical Science,Taian 271016,China;Shandong Jewim Pharmaceutical Co.,Ltd.,Taian 271000,China)

机构地区：[1]山东第一医科大学(山东省医学科学院),山东泰安271016 [2]山东京卫制药有限公司,山东泰安271000

出　　处：《山东化工》2024年第8期75-78,共4页Shandong Chemical Industry

基　　金：山东省高等学校大学生创新创业训练计划项目(S202210439002)。

摘　　要：以二硫化钼粉末为原料,N,N-二甲基甲酰胺(DMF)为溶剂,采用溶剂热法制备二硫化钼量子点(MoS_(2)QDs),并对其结构、组成和光学性质进行了表征。在此基础上,以MoS_(2)QDs为载体负载抗肿瘤药物盐酸阿霉素(DOX),构建了纳米载药体系(MoS_(2)-DOX),并通过体外药物释放实验和细胞毒性实验对其药物释放行为及治疗效果进行了初步评价。结果显示:MoS_(2)QDs分散均匀,尺寸约为3.0 nm,并具有良好荧光性质。借助MoS_(2)QDs自身的荧光性质,可追踪其进入U251胶质瘤细胞后主要存在于细胞质中。MoS_(2)-DOX具有明显的pH值响应性药物释放行为,其在酸性条件下(pH值为5.0和6.0)48 h的DOX释放率(35.1%和28.7%)明显高于pH值为7.4环境下的释放率(18.2%)。MoS_(2)QDs质量浓度低于150μg·mL^(-1)时对U251胶质瘤细胞无明显毒性;而MoS_(2)-DOX的细胞毒性呈现DOX剂量依赖性,且低于游离的DOX。这表明本方法制备的MoS_(2)QDs有望作为安全性较高的荧光纳米载体,在细胞成像和药物输送等领域具有广泛的应用前景。Molybdenum disulfide quantum dots(MoS_(2)QDs)were prepared by the solvothermal method using molybdenum disulfide powder as raw material and N,N-dimethylformamide(DMF)as a solvent,and their structure,composition,and optical properties were characterized.On this basis,a drug nanocarrier(MoS_(2)-DOX)was developed by using MoS_(2)QDs as carriers to load the anti-tumor drug doxorubicin hydrochloride(DOX).The drug release behavior and therapeutic effect of MoS_(2)-DOX were preliminarily evaluated through in vitro drug release and cytotoxicity experiments.The results show that the average particle size of MoS_(2)QDs is about 3.0 nm,and it has good dispersibility and fluorescence properties.By utilizing the fluorescence of MoS_(2)QDs,it can be traced that they mainly exist in the cytoplasm after entering U251 glioma cells.MoS_(2)-DOX exhibited significant pH-responsive drug release behavior.Under acidic conditions with pH=5.0 and 6.0,the DOX release rates of MoS_(2)-DOX at 48 hours were 35.1%and 28.7%,respectively,significantly higher than that at pH=7.4(18.2%).MoS_(2)QDs showed no significant toxicity to U251 glioma cells as their concentration was below 150μg·mL^(-1),while the cytotoxicity of MoS_(2)-DOX exhibited a dose-dependent effect on DOX,and its lethal effect on U251 glioma cells was lower than that of free DOX.This indicates that the MoS_(2)QDs prepared by this method are expected to be used as highly safe fluorescent nanocarriers and have broad application prospects in fields such as cell imaging and drug delivery.

关 键 词：二硫化钼量子点 溶剂热法 盐酸阿霉素 纳米载药体系 pH值响应药物释放 

分 类 号：TQ327.8[化学工程—合成树脂塑料工业]