板栗PAT基因家族成员鉴定及不同胁迫响应分析  

Identification of PAT gene family members and analysis of their response to different stresses in Chinese chestnut

作　　者：李金梅 聂兴华 葛婧怡 褚世慧 刘阳 秦岭[1] 邢宇[1] LI Jinmei;NIE Xinghua;GE Jingyi;CHU Shihui;LIU Yang;QIN Ling;XING Yu(College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]北京农学院植物科学技术学院,北京102206

出　　处：《果树学报》2024年第5期847-860,共14页Journal of Fruit Science

基　　金：国家自然科学基金项目(32271929)。

摘　　要：【目的】鉴定和分析板栗PAT基因家族及其对不同胁迫的响应,探究板栗PAT基因家族的抗逆功能。【方法】在板栗全基因组水平上进行搜索和鉴定板栗PAT基因家族成员,利用生物信息学方法研究其系统发育进化树、基因结构和motif、蛋白理化性质、染色体定位、共线性和启动子顺式元件等。以燕山红栗为试验材料,分析盐胁迫、抗病胁迫和干旱胁迫处理对板栗PAT基因家族表达模式的影响。【结果】在板栗基因组中共鉴定出包含DHHC结构域的21个PAT基因家族成员,他们与24个AtPAT基因家族成员共聚集为6个亚组;大多数CmPAT家族成员为具有亲水性的碱性稳定蛋白;21个PAT基因家族成员不均匀地分布在板栗的9条染色体上;在CmPAT基因启动子区域鉴定到多种非生物胁迫及激素响应元件;表达模式分析表明,多个CmPAT基因不同程度地参与抗病、干旱、盐胁迫响应。【结论】共鉴定了21个板栗PAT基因家族成员,同时筛选到CmPAT24、CmPAT7、CmPAT14可能共同参与了盐胁迫和干旱胁迫的调控,CmPAT7可能共同参与盐胁迫、干旱胁迫、抗病胁迫的调控。【Objective】The reversibility of protein palmitoylation is a key mechanism for regulating cellular function.Palmitoyl transferase is an important part of this mechanism,which can connect palmitic acid to the cysteine residue of the target protein.Protein palmitoylation is a post-transcriptional modification that plays a critical role in protein transport and function.Palmitate transferase(PAT)catalyzes the occurrence of acylation.Acylated PAT proteins may be involved in a variety of stress responses in material transport and signal transduction.Palmitate transferase plays an important role in growth,development and response to harmful stresses in plants.However,understanding of PAT genes in chestnut is limited so far.In the experiment,we performed a genome-wide identification of the PAT gene family in chestnut and subsequently analyzed how it responded to various stresses in Chinese chestnut.【Methods】HMMER,NCBI-CDD and SMART searches were performed to identify PAT genes throughout the chestnut genome.The domain information of the candidate gene family members was obtained through preliminary screening,and the protein sequences without the DHHC domain were eliminated.Ultimately,21 members of the chestnut PAT gene family were identified.The bioinformatics methods were used to study the phylogenetic tree,gene structure and gene motif,protein physicochemical properties,subcellular and chromosomal localization,collinearity and promoter cis-elements of the PAT genes.To check the stress-resistant function of PAT gene family,Yanshanhong cultivar was used as experimental material.For salt treatment,the seedling roots were submerged in a solution containing 200 mmol·L-1 NaCl and samples were collected on the 7th,10th and 14th days.Drought treatment was also carried out.Samples were collected and tested at different water loss in fresh leaves,specifically at 0%,5%,30%and 50%.To treat disease resistance,Chestnut Blight Mycelia Blocks were cultured on PAD medium.After 3 days,the PAD agar blocks containing chestnut bli

关 键 词：板栗 棕榈酰基转移酶(PAT) 胁迫处理 

分 类 号：S664.2[农业科学—果树学]