外源IAA对核桃内果皮生长发育的影响  

Effect of exogenous IAA on the growth and development of walnut endocarp

作　　者：李义霞 鱼尚奇 郭众仲 付嘉智 鹿宏丽 王红霞[4] 张锐 木塔力甫[1,2,3] LI Yixia;YU Shangqi;GUO Zhongzhong;FU Jiazhi;LU Hongli;WANG Hongxia;ZHANG Rui;Mutellip(The National-Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology on Characteristic Fruit Trees,Alar 843300,Xinjiang,China;Key Laboratory for the Protection and Utilization of Biological Resources in the Tarim Basin of Xinjiang Production and Construction Corps,Alar 843300,Xinjiang,China;College of Horticulture and Forestry,Tarim University,Alar 843300,Xinjiang,China;Hebei Institute of Mountain Research,Hebei Agricultural University,Baoding 071033,Hebei,China)

机构地区：[1]南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室,新疆阿拉尔843300 [2]新疆兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300 [3]塔里木大学园艺与林学学院,新疆阿拉尔843300 [4]河北农业大学河北省山区研究所,河北保定071033

出　　处：《果树学报》2024年第5期941-955,共15页Journal of Fruit Science

基　　金：国家自然科学基金项目(32160689);南疆重点产业创新发展支撑计划(2022DB022);“天山英才”培养计划。

摘　　要：【目的】探究生长素(IAA)对核桃内果皮发育的影响。【方法】采用不同质量浓度的IAA处理核桃,通过Wiesner法观察核桃内果皮木质素的沉积变化,并测定内果皮中木质素、纤维素、半纤维素和IAA含量,同时对IAA处理后第12天的样本进行高通量测序。【结果】与对照相比,低质量浓度IAA处理和高质量浓度IAA处理后内果皮木质素的积累存在显著差异,在处理后期,核桃内果皮部分硬化,仍存在内果皮缺失或不完整现象。木质素、纤维素和半纤维素含量整体呈上升趋势,最高分别可达30.55%、16.49%和17.23%,IAA含量整体呈上升趋势;通过RNA-seq分析,发现差异基因主要富集在植物激素信号转导和苯丙烷生物合成途径中,且随着IAA质量浓度的升高,部分与激素信号转导和苯丙烷生物合成相关的差异表达基因逐渐下调表达或不表达。【结论】IAA参与调控核桃内果皮的发育,影响内果皮木质素的积累,为后期研究核桃内果皮对外源生长素的响应提供参考依据。【Objective】The accumulation of lignin is the main events of walnut endocarp development,and plant hormones regulate the formation of thick-walled tissues and the synthesis of lignin,which involves in xylem development,through the expression of related genes,thus affecting the development of the secondary wall.The study was carried out in order to understand the effect of the growth hormone IAA on the development of endocarp in walnuts.【Methods】In the present study,we selected the 16 year old trees with robust and consistent growth and consistent levels of water and fertilizer management.At about 50 d after flowering,bearing spurs were wrapped and treated with different concentrations of growth regulators.Changes in lignin deposition in the endocarp of walnuts after the treatments with different concentrations of IAA was observed by resorcinol staining,and the contents of lignin,cellulose,hemicellulose,and IAA in the endocarp were determined.At the same time,samples at 12 d after IAA treatment were subjected to high-throughput sequencing and analysis.【Results】The results showed that at the early stage of treatment there was a significant difference in the accumulation of endocarp lignin between the treatments with differential IAA concentrations.Lignin deposition started from tissues near the vascular bundles,and the color shifted from light red to dark red and from localized staining to overall staining.At the late stage,the endocarp of the late walnuts was partially hardened and significantly thickened,but the development of the endocarp was incomplete,and the dewy kernel was still present.Lignin content in the endocarp increased significantly after the treatments with 50 mg·L^(-1) IAA and 100 mg·L^(-1) IAA at the 12th d after treatment,and the difference in lignin content was not significant between the control and the treatment with 200 mg·L^(-1).In the late stages,low concentration IAA treatment promoted lignin accumulation and lignin content increased slowly,but lignin content in treatments hi

关 键 词：核桃 内果皮 IAA 木质素 高通量测序 

分 类 号：S664.1[农业科学—果树学]