东北大黑鳃金龟幼虫高毒力球孢白僵菌菌株的筛选及应用  

Screening and Application of High Toxicity Beauveria bassiana Strains against Holotrichia diomphalia Larvae

作　　者：张强[1] 张云月[1] 朱晓敏[1] 路杨[1] 常雪[1] 高悦 赫思聪 李茂海[1] 田志来[1] ZHANG Qiang;ZHANG Yunyue;ZHU Xiaomin;LU Yang;CHANG Xue;GAO Yue;HE Sicong;LI Maohai;TIAN Zhilai(Institute of Plant Protection,Jilin Academy of Agricultural Science/Jilin Key Laboratory of Agricultural Microbiology/Key Laboratory of Integrated Pest Management on Crops in Northeast China,Ministry of Agriculture and Rural Affairs,Gongzhuling 136100,China;Jilin Jixing Agricultural Technical Service Center,Gongzhuling 136100,China)

机构地区：[1]吉林省农业科学院植物保护研究所/吉林省农业微生物重点实验室/农业农村部东北作物有害生物综合治理重点实验室,公主岭136100 [2]吉林省吉兴农业技术服务中心,公主岭136100

出　　处：《中国生物防治学报》2024年第2期291-298,共8页Chinese Journal of Biological Control

基　　金：吉林省农业科技创新工程(CXGC2021ZY021);吉林省科技厅重点研发(20210202122NC);吉林省科技厅重点研发(20210202092NC)。

摘　　要：为了筛选到针对靶标害虫东北大黑鳃金龟幼虫高毒力球孢白僵菌菌株,通过室内生物测定,明确球孢白僵菌Bb QCGZL-1对东北大黑鳃金龟幼虫的致病力,并与其他药剂进行防效对比;同时通过盆栽试验测定了球孢白僵菌BbQCGZL-1在不同农作物上对东北大黑鳃金龟幼虫的防治效果。球孢白僵菌BbQCGZL-1在施药14d时校正死亡率达到75%以上,高于其他供试菌株;施药7d时LC50为1.21×10^(7)cfu/mL,当菌悬液浓度为1.0×10^(8)cfu/mL时,LT_(50)为8.82 d;室内生测试验和室外盆栽试验结果表明,在东北地区利用球孢白僵菌防治东北大黑鳃金龟幼虫时,球孢白僵菌Bb QCGZL-1与商品化的微生物源药剂在防治效果上无显著差异。球孢白僵菌BbQCGZL-1可作为防治东北大黑鳃金龟幼虫的有效微生物源药剂。The aim of this study was to screen for highly toxicity Beauveria bassianan strains against the larvae of Holotrichia diomphalia Bates.The pathogenicity of a B.bassiana strain BbQCGZL-1 against the white grubs was determined by laboratory bioassay and its control efficiency against the grubs was compared to other agents.Further,the control efficiency of B.bassiana BbQCGZL-1 against the white grubs on different crops was tested by using outdoor potted plants.The results showed that the corrected mortality rate of the grubs was more than 75%at 14 d post treatment with the B.bassiana BbQCGZL-1,higher than those obtained with other stains tested;The LC_(50) was 1.21×10^(7) cfu/mL at 7 d post treatment,the LT50 was 8.82 d with a concentration of spore suspension of 1.0×10^(8) cfu/mL.The results of the laboratory bioassay and the outdoor potted plant experiment showed no significant difference in the control efficiency between B.bassiana BbQCGZL-1 and commercial biological agents against the white grubs in Northeast of China.Overall,the B.bassiana BbQCGZL-1 can be used as a microbial agent to control H.diomphalia larvae in the field.

关 键 词：东北大黑鳃金龟 蛴螬 球孢白僵菌 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]