拮抗放线菌2-1-2F的鉴定及其对黄瓜枯萎病的防效  

Identification of Antagonistic Actinomycetes 2-1-2F and Its Control Effect on Cucumber Fusarium Wilt

作　　者：蒋冬阳 吴冰越 季文平 陈宸 陈夕军 JIANG Dongyang;WU Bingyue;JI Wenping;CHEN Chen;CHEN Xijun(College of Plant Protection,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学植物保护学院,扬州225009

出　　处：《中国生物防治学报》2024年第2期411-423,共13页Chinese Journal of Biological Control

基　　金：现代农业产业技术体系项目(CARS-24-C-01)。

摘　　要：为获得可高效防控黄瓜枯萎病的生防放线菌,采用梯度稀释和平板对峙法从蔬菜根际土壤中筛选到1株对黄瓜枯萎病菌Fusarium oxysporum f.sp.cucumerinm(FOC)有较强拮抗作用的放线菌2-1-2F;经形态学和分子生物学鉴定,确定菌株2-1-2F为弗吉尼亚链霉菌Streptomycesvirginiae。其能产生蛋白酶、β-葡聚糖酶和几丁质酶,不产生嗜铁素和纤维素酶,且菌株2-1-2F产生的发酵滤液活性易受环境条件影响。菌株2-1-2F发酵菌液对黄瓜有明显的促生作用,可诱导黄瓜体内防御酶活性和抗性相关物质含量增加,并于处理后1~7 d内达最大值。菌株2-1-2F在黄瓜根部有较强的定殖能力,42 d后在土壤中仍可达10^(6)cfu/g;其对黄瓜枯萎病的预防和治疗效果分别达48.98%和34.01%。In order to obtain biocontrol actinomycetes to control cucumber Fusarium wilt,a strain 2-1-2F with strong antagonistic activity against F.oxysporum f.sp.cucumerinum(FOC)was isolated from vegetable rhizospheric soil by gradient dilution and confrontation culture methods.Strain 2-1-2F was identified as Streptomyces virginiae by morphology observation and molecular biology identification.It could produce proteases,chitinases andβ-glucanases,but not produce siderophores and cellulases.The activity of fermentation liquid produced by 2-1-2F was susceptible to environmental conditions.Fermentation liquid of 2-1-2F had obvious growth-promoting effect on cucumber plants,and could induce the cucumber defense enzyme activities and resistance related substance contents,which reached the maximum value from one to seven days after treatment.Strain 2-1-2F had strong colonization ability in cucumber roots,and the concentration of colonization could still reach 10^(6)cfu/g soil after 42 days.The preventive and therapeutic effects of 2-1-2F on cucumber Fusarium wilt were 48.98%and 34.01%,respectively.Actinomycetes 2-1-2F and its metabolites had the potential to be developed into biocontrol agents.

关 键 词：弗吉尼亚链霉菌 鉴定 黄瓜枯萎病 生防效果 

分 类 号：S476[农业科学—农业昆虫与害虫防治]