生防菌Y26的鉴定、发酵优化及其杀线虫活性测定  

Identification,Fermentation Optimization and Determination of Nematicidal Activity of Biocontrol Bacterium Y26

作　　者：梅凤珍 周明超 杨丹 李香风[5] 孔令安[3] 刘建斌[4] 史凤玉 MEI Fengzhen;ZHOU Mingchao;YANG Dan;LI Xiangfeng;KONG Lingan;LIU Jianbin;SHI Fengyu(College of Agronomy and Biotechnology,Hebei Normal University of Science and Technology,Changli 066004,China;Key Laboratory of crop stress biology,Hebei Normal University of Science and technology,Changli 066004,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Institute of Plant Nutrition,Resources and Environment,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Handan Garden Bureau of Hebei Province,Handan 056000,China)

机构地区：[1]河北科技师范学院农学与生物科技学院,昌黎066004 [2]河北科技师范学院河北省作物逆境生物学重点实验室,昌黎066004 [3]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193 [4]北京市农林科学院植物营养与资源环境研究所,北京100097 [5]河北省邯郸市园林局,邯郸056000

出　　处：《中国生物防治学报》2024年第2期424-434,共11页Chinese Journal of Biological Control

基　　金：河北省重点研发计划项目(21326502D);植物病虫害生物学国家重点实验室开放基金(SKL0F202115)。

摘　　要：为防治黄瓜根结线虫病,本实验室前期从黄瓜根际土壤分离出的一株细菌Y26,测定其发酵液对根结线虫的生物活性,经发酵液处理后的根结线虫校正死亡率为95.12%,卵孵化的相对抑制率为83.51%,故菌株Y26可作为根结线虫生防菌资源;通过形态学观察及分子生物学方法鉴定菌株Y26为短小芽胞杆菌Bacillus pumilus;通过Plackett-Burma法和响应面分析试验得到菌株Y26发酵优化的条件是:玉米粉2.14%,豆饼粉3%,K_(2)HPO_(4)0.57%,MgSO_(4)·7H_(2)O 0.21%,种龄12 h,pH为7.2,接种量2%,培养温度28℃,装瓶量为50 mL/250 mL,发酵优化后,菌液中芽胞浓度提高25.69%,10%发酵液对根结线虫的校正死亡率从优化前的78.03%提高到93.32%。研究结果表明,经发酵优化后的Y26菌液杀线虫活性显著提高,可作为杀线生物菌剂开发应用。To control the root-knot nematode disease in cucumber,a bacterial strain Y26 was isolated from cucumber rhizosphere soil in the laboratory.Bio-activity of fermentation broth against root-knot nematodes was determined.The corrected mortality rate of the second-stage juveniles treated with fermentation broth for 24 hours was 95.12%,and the relative inhibition rate of eggs hatching reached 83.51%after 48 hours of fermentation broth treatment,so strain Y26 can be used as a bacterial control resource for root-knot nematodes disease.Strain Y26 was identified as Bacillus pumilus by morphological observation and molecular identification.The optimized conditions for fermentation of strain Y26 by Plackett-Burma design and response surface analysis were corn flour 2.14%,bean cake flour 3%,K_(2)HPO_(4)0.57%,MgSO_(4)·7H_(2)O 0.21%,cultural time 12 hours,pH 7.2,inoculation amount 2%,cultural temperature 28℃,and the media amount was 50 mL/250 mL.After fermentation optimization,the spore concentration was increased by 25.69%,the corrected mortality rate of 10%fermentation broth increased from 78.03%to 93.32%before optimization.The results showed that the activity of optimized Y26 fermentation broth root-knot nematodes was significantly increased and could be developed and applicated as a biological nematicide.

关 键 词：根结线虫 短小芽胞杆菌 鉴定 发酵条件优化 

分 类 号：S476[农业科学—农业昆虫与害虫防治]