玉屏风多糖复合益生菌对大口黑鲈响应迟缓爱德华氏菌脂多糖的免疫调节作用  

Immunomodulatory effect of Yupingfeng polysaccharide compound probiotics on largemouth bass in response to Edwardsiellatarda lipopolysaccharide

作　　者：杨映[1] 杨颖 刘念 欧阳俊烨 赖明建 曾志华 于辉[1] YANG Ying;YANG Ying;LIU Nian;OUYANG Junye;LAI Mingjian;ZENG Zhihua;YU Hui(Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding,Foshan University,Foshan 528231,Guangdong,China;School of Life Science and Engineering,Foshan University,Foshan 528231,Guangdong,China;Foshan Institute of Agricultural Sciences,Foshan 528145,Guangdong,China)

机构地区：[1]佛山科学技术学院,广东省动物分子设计与精准育种重点实验室,广东佛山528231 [2]佛山科学技术学院生命科学与工程学院,广东佛山528231 [3]佛山市农业科学研究所,广东佛山528145

出　　处：《淡水渔业》2024年第3期69-77,共9页Freshwater Fisheries

基　　金：广东省动物分子设计与精准育种重点实验室(2019B030301010);广东省重点领域研发计划(2019B110209005)。

摘　　要：为探究玉屏风多糖复合益生菌对大口黑鲈(Micropterus salmoides)响应迟缓爱德华氏菌(Edwardsiellatarda)脂多糖(LPS)的免疫调节作用,选取450尾健康大口黑鲈(11±2)g,随机分为对照组、复合益生菌组(CPB)、玉屏风多糖组(YPF)、未发酵玉屏风多糖复合益生菌组(UYP)和发酵玉屏风多糖复合益生菌组(FYP)5组,饲喂28 d后注射迟缓爱德华氏菌LPS,在注射后0、8、24、48 h采样,测定免疫器官生长指数、血清生化指标、组织抗氧化指标和肝脏免疫相关基因表达。结果显示:饲喂28 d后,FYP组头肾指数显著高于对照组和YPF组。在LPS免疫刺激0 h时,实验组血清总抗氧化能力均显著高于对照组。免疫刺激8 h时UYP组和FYP组血清谷草转氨酶和谷丙转氨酶活性显著高于对照组,肝脏白细胞介素-1和肿瘤坏死因子-α浓度显著高于对照组,肝脏核因子-κB抑制物激酶、白细胞介素-1β、白细胞介素-8相对表达量较对照组均显著下调。与其他实验组相比,FYP组在免疫刺激后8 h时肠组织和肝组织超氧化物歧化酶活性显著高于其他组,48 h时血清免疫球蛋白M浓度显著高于其他组。综上,本研究表明玉屏风多糖与复合益生菌均能提高大口黑鲈的免疫能力,发酵玉屏风多糖复合益生菌效果更佳。A total of 450 healthy largemouth bass(11±2)g were selected to investigate Yupingfeng polysaccha-ride complex probiotics on the delayed Edwardsiellatarda lipolysaccharides of Micropterus salmoides.They were ran-domly divided into a control group,compound probiotic group(CPB),YPF group,unfermented compound probiotic group(UYP)and fermented compound probiotic group(FYP),and fed 28 days later were injected with LPS of Edwardsiella tarda,and samples were collected at 0,8,24 and 48 h after injection.Immune or-gan growth index,serum biochemical index,tissue antioxidant index and liver immune-related gene expression were measured.The results showed that the head kidney index in FYP group was significantly higher than that in control group and YPF group after feeding for 28 days.After 0 h of LPS immunostimulation,the serum total antioxidant capacity of the experimental group was significantly higher than that of the control group.After 8 h of immunostimulation,serum glutamic oxalacetic transaminase and glutamic pyruvic transaminase activities in UYP and FYP groups were significantly higher than those in control group,liver interleukin-1 and tumor nec-rosis factor-αconcentrations were significantly higher than those in control group,and the relative expressions of nuclear factor-κB inhibitor kinase,interleukin-1βand interleukin-8 in liver were significantly down-regula-ted.Compared with other experimental groups,the superoxide dismutase activity in intestinal tissue and liver tis-sue in FYP group was significantly higher than that in different groups at 8 h after immune stimulation,and the serum IgM concentration at 48 h was significantly higher than in other groups.In conclusion,this study showed that both Yupingfeng polysaccharide and compound probiotics could improve the immune capacity of Microp-terus salmoides,and the effect of fermented Yupingfeng polysaccharide compound probiotics was better.

关 键 词：大口黑鲈(Micropterus salmoides) 玉屏风多糖 复合益生菌 免疫 

分 类 号：S965.199[农业科学—水产养殖]