淡附片标准汤剂质量标准研究  

Study on the Quality Standard of Desalted Aconiti Lateralis Radix Praeparata Standard Decoction

作　　者：申屠乐 李影影 裴亚军 缪媛媛 SHENTU Le;LI Yingying;PEI Yajun;MIAO Yuanyuan(The Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005,China;Zhejiang Tiandao Pharmaceutical Co.Ltd.,Hangzhou 310000,China)

机构地区：[1]浙江中医药大学附属第二医院,浙江杭州310005 [2]浙江天道医药有限公司,浙江杭州310000

出　　处：《中国中医药信息杂志》2024年第6期136-140,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：浙江省中医药科技计划(2017ZKL012、2023ZF111)。

摘　　要：目的制备淡附片标准汤剂,进行含量测定及指纹图谱分析。方法制备15批淡附片标准汤剂,通过检测单酯型生物碱的含量计算其转移率;采用HPLC建立淡附片标准汤剂指纹图谱,计算指纹图谱相似度。结果15批淡附片标准汤剂的单酯型生物碱转移率为56.93%~94.06%,平均转移率为78.03%。经HPLC指纹图谱分析,发现共有峰9个,确认其中3个;15批淡附片标准汤剂的指纹图谱与对照指纹图谱相似度均大于0.95。结论本研究建立了淡附片标准汤剂HPLC含量测定和指纹图谱分析方法,可为淡附片中药配方颗粒的质量控制提供参考。Objective To prepare desalted Aconiti Lateralis Radix Praeparata(Danfupian)standard decoction for HPLC content determination and fingerprint analysis.Methods 15 batches of Danfupian standard decoction were prepared,and the transfer rate of monoester alkaloids was calculated by detecting their content.The transfer rates of monoester alkaloids of 15 batches of Danfupian standard decoction were calculated.The fingerprints of Danfupian standard decoction were established by HPLC,and the similarity values of fingerprints were calculated.Results According to the measurement of 15 batches of Danfupian standard decoction,the average transfer rate of monoester alkaloids was 78.03%with a rage from 56.93%to 94.06%.Besides,9 common peaks were determined and 3 peaks were calibrated.Moreover,the similarity was over 0.95 between fingerprints of standard decoction and the control fingerprint of the 15 batches of Danfupian standard decoction.Conclusion This study establishes an HPLC content determination and fingerprint analysis method of Danfupian standard decoction,which can provide reference for the quality control of the TCM granules of Danfupian.

关 键 词：淡附片 标准汤剂 高效液相色谱法 含量测定 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]