影响绵羊卵母细胞回收效率因素的研究  

Effect of Different Methods of Oocyte Retrieval in Sheep

作　　者：梁红艳 陈莹 张欣如 马秀玲 胡凯 刘宽宽 白新宇 林嘉鹏 吴阳升 刘书东[1] 汪立芹 Liang Hongyan;Chen Ying;Zhang Xinru;Ma Xiuling;Hu Kai;Liu Kuankuan;Bai Xinyu;Lin Jiapeng;Wu Yangsheng;Liu Shudong;Wang Liqin(College of Animal Science and Technology,Tarim University,Alar,843300,Xinjiang;Key Laboratory of Animal Biotechnology of Xinjiang,Key Laboratory of Genetics Breeding and Reproduction of Grass Feeding Livestock,Ministry of Agriculture and Rural Affairs,Biotechnology Research Institute,Xinjiang,Institute of Animal Biotechnology,Xinjiang Academy of Animal Science,Urumqi,Xinjiang 830011,China;College of Animal Science and Technology,Xinjiang Agricultural University,Urumqi,830091,Xinjiang)

机构地区：[1]塔里木大学动物科学与技术学院,新疆阿拉尔843300 [2]新疆动物生物技术重点实验室/农业农村部草食家畜遗传育种与繁殖重点实验室/新疆畜牧科学院生物技术研究所,新疆乌鲁木齐830011 [3]新疆农业大学动物科学学院,新疆乌鲁木齐830052

出　　处：《中国畜禽种业》2024年第4期109-116,共8页The Chinese Livestock and Poultry Breeding

基　　金：自治区科技援疆项目(2022E02017);科技创新2030-农业生物育种重大项目(2022ZD04013);自治区重大专项(2023A02011);自治区“两区”科技发展计划(2022LQ04006)。

摘　　要：为优化卵母细胞采集方法、提高绵羊卵巢资源利用率奠定基础,该试验利用屠宰绵羊卵巢,研究采卵人员及采卵工具(注射器、负压泵)对卵母细胞回收效率的影响。结果表明:不同人员对卵母细胞回收数量和质量均存在极显著差异(P<0.01);哈萨克羊/阿勒泰羊卵母细胞回收数量和质量显著低于湖羊(P<0.05);不同规格注射器针头,对绵羊卵母细胞回收数量和质量存在极显著差异(P<0.01),且9号针头(兽用)采卵效果较优;真空负压泵对卵母细胞的回收效果显著高于注射器(P<0.05)。可见,注射器采集卵母细胞对技术人员的熟练程度要求较高,采用真空负压泵采卵方法卵母细胞回收效率显著高于注射器采卵方法。In order to optimize the oocyte collection method and improve the utilization rate of ovarian resources in sheep,this study studied the effects of oocyte collection personnel and oocyte collection tools(syringe,negative pressure pump)on the efficiency of oocyte recovery in slaughtered sheep ovaries.The results showed that:There were significant differences in the quantity and quality of oocytes retrieved by different personnel(P<0.01).The quantity and quality of oocytes recovered in Kazakh sheep or Altay sheep were significantly lower than Hu sheep(P<0.05).There were significant differences in the quantity and quality of oocytes recovered by different syringe needles(P<0.01),and the 9 needle(veterinary use)had the best effect.The recovery effect of vacuum negative pressure pump on oocytes was significantly higher than that of syringe(P<0.05).It can be concluded that oocyte collection by syringe requires a high level of proficiency of technicians.The oocyte recovery efficiency of vacuum negative pressure pump oocyte collection was significantly higher than that of syringe oocyte collection.

关 键 词：绵羊 卵母细胞 真空负压泵 采集方法 回收率 

分 类 号：S826[农业科学—畜牧学]