正交试验优选藏药肺热普清配方中水溶性有效成分提取工艺  

Optimization of extraction process of water-soluble active ingredients in the formula of Tibetan medicine Lotse Kunsel by orthogonal test

作　　者：宋丽莉 采金金 尼玛次仁 白玛旦增 谢和兵 SONG Lili;CAI Jingjing;NIMA Ciren;BAIMA Danzeng;XIE Hebing(Jiangsu God Monkey Pharmaceutical Research Co.,Ltd.,Nantong 226000,China;School of Pharmacy,Nantong University,Nantong 226000,China;School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Yangtze Delta Drug Advanced Research Institute,Nantong 226000,China;Tibet God Monkey Pharmaceutical Co.,Ltd.,Shigatse 858300,China)

机构地区：[1]江苏神猴医药研究有限公司,南通226000 [2]南通大学药学院,南通226000 [3]安徽中医药大学药学院,合肥230012 [4]长三角药物高等研究院,南通226000 [5]西藏神猴药业有限责任公司,日喀则858300

出　　处：《上海医药》2024年第9期122-126,共5页Shanghai Medical & Pharmaceutical Journal

基　　金：西藏自治区科技厅中央引导地方科技发展项目(XZ202202YD0020C)。

摘　　要：目的:优选肺热普清配方中水溶性有效成分的提取工艺。方法:采用L_(9)(3^(4))正交试验法优选提取工艺,以干浸膏得率、羟基红花黄色素A转移率、乙酸乙酯萃取粗品得率为指标,考察加水倍数、提取时间、提取次数3个因素对提取工艺的影响。结果:优选的最佳工艺为加12倍水,提取3次,每次1 h。结论:优选的工艺稳定可靠,有效成分转移率高。Objective:To optimize the extraction process of water-soluble active ingredients in the formula of Lotse Kunsel.Methods:The yield of dry extract,the transfer rate of hydroxysafflower yellow A and the yield of solid content extracted by ethyl acetate were used as indexes.The effects of water addition ratio,extraction time and extraction times on the extraction process were investigated.L_(9)3^(4) orthogonal test was used to optimize the extraction process.Results:The best process was to add 12 times of water and extract three times for 1 hour each.Conclusion:The optimized process is stable and reliable and the transfer rate of active ingredients is high.

关 键 词：肺热普清 提取工艺 正交试验 羟基红花黄色素A 

分 类 号：R284.2[医药卫生—中药学] R29[医药卫生—中医学]