二氢杨梅素对高糖刺激下心肌细胞坏死性凋亡的影响  

The effects of dihydromyricetin on necroptosis in high glucose-stimulated cardiomyocytes

作　　者：孙琳琳[1] 施益金 施卉 陈万杰 顾锦华[1] SUN Linlin;SHI Yijin;SHI Hui;CHEN Wanjie;GU Jinhua(Department of Pharmacy,Affiliated Maternity&Child Health Care Hospital of Nantong University,Nantong 226007,Jiangsu,China)

机构地区：[1]南通大学附属妇幼保健院药学部

出　　处：《医学研究与战创伤救治》2024年第2期130-136,共7页Journal of Medical Research & Combat Trauma Care

基　　金：南通市科技计划项目(MSZ2022097);南通市卫生健康委员会科研课题计划资助(QNZ2023072)。

摘　　要：目的探究二氢杨梅素(DHY)对高糖刺激下心肌细胞损伤的影响。方法原代心肌细胞用DHY(80μmol/L预处理4 h,用正常葡萄糖(NG)5.5 mmol/L或高糖(HG)33.3 mmol/L刺激48 h。随后利用MTT法评估细胞活性,测定乳酸脱氢酶(LDH)评估细胞损伤程度,二氢乙锭(DHE)和MitoSOX染色评估氧化应激水平,蛋白印迹和免疫荧光检测受体相互作用蛋白激酶3(RIPK3)蛋白表达,原位末端转移酶标记(TUNEL)法检测心肌细胞凋亡程度。结果与HG组相比,DHY显著增强高糖刺激下心肌细胞活性(P=0.003),减少LDH释放(P=0.001),减弱DHE和MitoSOX染色荧光强度,显著抑制RIPK3蛋白表达(P<0.01),减少TUNEL染色阳性细胞数量(P<0.01)。结论DHY增强高糖刺激下心肌细胞活性,减轻损伤程度,可能与DHY抑制氧化应激和减弱坏死性凋亡有关。Objective To investigate the effects of dihydro myricetin in high glucose-stimulated cardiomyocytes injury.Methods Primary cardiomyocytes were pretreated with DHY(80μmol/L)for 4 h,followed by normal glucose(NG,5.5 mmol/L)or high glucose(HG,33.3 mmol/L)stimulation for 48 h.Subsequently,cell viability was assessed by MTT.The degree of cell damage was evaluated by lactate dehydrogenase(LDH)measurement.The level of oxidative stress was assessed with dihydroethidium(DHE)and MitoSOX staining.The protein expression of receptor interacting protein kinase 3(RIPK3)was detected by immunofluorescence and Western blot.The degree of cardiomyocytes apoptosis was determined by TdT-mediated dUTP nick end labeling(TUNEL).Results Compared with HG group,DHY significantly enhanced cardiomyocytes activity(P=0.003),reduced LDH release(P=0.001),alleviated the fluorescence intensity of DHE and MitoSOX staining,inhibited RIPK3 protein expression(P<0.01),and reduced the number of positive cells with TUNEL staining(P<0.01)in high glucose-stimulated cardiomyocytes.Conclusion DHY increased cardiomyocytes viability and attenuated cell injury,which might be related to oxidative stress inhibition and necroptosis attenuating in high glucose-stimulated cardiomyocytes.

关 键 词：二氢杨梅素 高糖 氧化应激 受体相互作用蛋白激酶3 

分 类 号：R392[医药卫生—免疫学]