血清lncRNA DUXAP8及miR-24-3p在子痫前期诊断及妊娠结局评估中的价值  

Value of serum lncRNA DUXAP8 and miR-24-3p in the diagnosis of preeclampsia and the evaluation of pregnancy outcomes

作　　者：高海侠[1] 高京京 张晓月 司凡[1] 刘晓铮 刘双双 GAO Haixia;GAO Jingjing;ZHANG Xiaoyue;SI Fan;LIU Xiaozheng;LIU Shuangshuang(Department of Obstetrics,Women's and Children's Hospital,Chengde Central Hospital,Chengde 067000,Hebei,China)

机构地区：[1]承德市中心医院妇儿院区产科,承德067000

出　　处：《医学研究与战创伤救治》2024年第2期155-160,共6页Journal of Medical Research & Combat Trauma Care

基　　金：承德市科技计划自筹经费项目(202301A018)。

摘　　要：目的分析血清长非编码RNA双同源盒A伪基因8(lncRNA DUXAP8),微小RNA(miR)-24-3p在子痫前期(PE诊断及妊娠结局评估中的价值。方法选取2019年3月至2022年9月承德市中心医院妇儿院区产科124例PE患者为PE组,同期健康孕妇124例为对照组。根据妊娠结局分为不良组27例和良好组97例。均采用qRT-PCR法测定血清lncRNA DUXAP8、miR-24-3p水平。采用多因素Logistic回归分析PE孕妇妊娠结局影响因素。采用ROC曲线分析血清lncRNA DUXAP8、miR-24-3p水平诊断PE及预后的价值。结果PE组血清lncRNA DUXAP8水平低于对照组,miR-24-3p水平高于对照组(P<0.05)。TargetScanHuman网站预测显示,lncRNA DUXAP8与miR-24-3p间可能存在靶向关系。相关性分析表明,lncRNA DUXAP8与miR-24-3p呈负相关(r=-0.471,P<0.001)。血清lncRNA DUXAP8、miR-24-3p联合诊断PE的AUC显著高于单独诊断(Z_(lncRNA DUXAP8-联合)=3.285,P=0.001;Z_(miR-24-3p-联合)=3.020,P=0.003),联合诊断的敏感性为92.74%,特异性为72.31%。良好组孕妇血清lncRNA DUXAP8水平高于不良组,miR-24-3p水平低于不良组(P<0.05)。良好组孕妇收缩压、舒张压、产前血糖、血红蛋白、24 h尿蛋白、白细胞计数、LDH低于不良组,血小板计数、血清白蛋白高于不良组(P<0.05)。lncRNA DUXAP8是PE孕妇妊娠结局不良保护因素,miR-24-3p是危险因素(P<0.05)。血清lncRNA DUXAP8、miR-24-3p联合诊断PE孕妇妊娠结局的AUC显著高于单独诊断(Z_(lncRNA DUXAP8-联合)=2.113,P=0.035,Z_(miR-24-3p-联合)=3.033,P=0.002),联合诊断的敏感性为88.89%,特异性为80.41%。结论PE孕妇lncRNA DUXAP8水平降低,miR-24-3p水平升高,可能与PE的发生和孕妇妊娠结局相关。Objective To analyze the value of serum long non-coding RNA dual homeobox A pseudogene 8(lncRNA DUXAP8)and microRNA(miR)-24-3p in the diagnosis of preeclampsia(PE)and the evaluation of pregnancy outcomes.Methods From March 2019 to September 2022,124 PE patients in the Department of Obstetrics and Gynecology of Chengde City Central Hospital of Hebei Province were collected as the PE group,and meanwhile 124 healthy pregnant women were included as the control group.According to pregnancy outcomes,they were categorized into an adverse-outcome group of 27 cases and a good-outcome group of 97 cases.qRT-PCR method was applied to determine serum lncRNA DUXAP8 and miR-24-3p levels.Multivariate Logistic regression was applied to analyze the influencing factors of pregnancy outcomes in PE pregnant women.ROC curve was applied to analyze the diagnostic and prognostic value of serum lncRNA DUXAP8 and miR-24-3p levels in PE.Results The serum lncRNA DUXAP8 level in the PE group was lower than that in the control group,and the miR-24-3p level was higher than that in the control group(P<0.05).Target Scan Human website predicted that there may be a targeting relationship between lncRNA DUXAP8 and miR-24-3p.Correlation analysis showed a negative correlation between lncRNA DUXAP8 and miR-24-3p(r=-0.471,P<0.001).The AUC of serum IncRNA DUXAP8 combined miR-24-3p in the diagnosis for PE was obviously higher than that of single diagnosis(Z_(lncRNA DUXAP8-combination)=3.285,P=0.001,Z_(miR-24-3p-combination)=3.020,P=0.003),and the sensitivity of combined diagnosis was 92.74%,and the specificity was 72.31%.The serum lncRNA DUXAP8 level in the good-outcome group was higher than that in the adverse-outcome group,and the miR-24-3p level was lower than that in the adverse-outcome group(P<0.05).The systolic blood pressure,diastolic blood pressure,prenatal blood glucose,hemoglobin,24-hour urine protein,white blood cell count,and LDH in the good-outcome group were lower than those in the adverse-outcome group,while platelet count and serum albumin

关 键 词：长非编码RNA双同源盒A伪基因8 微小RNA-24-3p 子痫前期 妊娠结局 

分 类 号：R714.2[医药卫生—妇产科学]