血清MMP-7、ADAM17在结缔组织病相关间质性肺病中的表达及相关性研究  

The Expression and Correlation of Serum MMP-7 and ADAM17 in Connective Tissue Disease-related Interstitial Lung Disease

作　　者：孙英梅 闫慧明[3] SUN Ying-mei;YAN Hui-ming

机构地区：[1]内蒙古科技大学包头医学院,内蒙古包头014040 [2]乌海市人民医院,内蒙古乌海016000 [3]内蒙古科技大学包头医学院第二附属医院,内蒙古包头014030

出　　处：《风湿病与关节炎》2024年第5期20-26,共7页Rheumatism and Arthritis

摘　　要：目的:探讨基质金属蛋白酶-7(MMP-7)、解整合素金属蛋白酶17(ADAM17)在结缔组织病(CTD)相关间质性肺病(ILD)中的表达水平及相关性研究。方法:收集2021年12月至2023年7月在包头医学院第一及第二附属医院风湿免疫科诊治的CTD-ILD患者40例为CTD-ILD组,不合并ILD的CTD患者30例为CTD组,以及26例同期健康体检者为对照组,收集患者基本资料,酶联免疫吸附法(ELISA)检测3组血清MMP-7、ADAM17的表达水平;按一氧化碳弥散量占预计值的百分比[DLCO(%pred)]将CTD-ILD分为轻度、中度和重度,分析CTD-ILD不同亚组、不同肺功能组血清MMP-7、ADAM17的表达水平,阐释MMP-7、ADAM17与各指标的相关性及评估它们在诊断CTD-ILD中的价值。结果:①CTD-ILD组与CTD组、对照组比较,血清MMP-7、ADAM17显著升高(P<0.01);②CTD-ILD不同亚组中,与SS-ILD组比较,血清MMP-7、ADAM17水平在SSc-ILD组中较高(P<0.05);③CTD-ILD不同肺功能组中,血清MMP-7水平为重度>中度>轻度(P<0.01),血清ADAM17水平在中、重度组>轻度组(P<0.01);④相关性分析得出,CTD-ILD患者中MMP-7与CT评分、CPI及年龄呈正相关(P<0.05),ADAM17与CT评分、CPI呈正相关(P<0.01),MMP-7、ADAM17与第一秒用力呼吸容积、用力肺活量、DLCO(%pred)、P(O_(2))均呈负相关(P<0.05)。结论:血清MMP-7、ADAM17可能参与了CTD-ILD的发生、发展,对CTD-ILD病情严重程度的评估有一定的预测价值。Objective:To investigate the expression level and correlation of matrix metalloproteinase-7(MMP-7)and a disintegrin and metalloproteinase 17(ADAM17)in connective tissue disease(CTD)-related interstitial lung disease(ILD).Methods:A total of 40 CTD-ILD patients treated in the Rheumatology and Immunology Department of the First and Second Affiliated Hospital of Baotou Medical College from December 2021 to July 2023 were collected as the CTD-ILD group,30 CTD patients without ILD as the CTD group,and 26 healthy individuals who underwent physical examinations during the same period as the control group.Basic information of patients was collected,and the expression levels of MMP-7 and ADAM17 in serum were detected by enzyme-linked immunosorbent assay(ELISA)in the three groups;CTD-ILD was classified into the mild,the moderate,and the severe according to the percentage of carbon monoxide diffusion to the expected value[DLCO(%pred)].The expression levels of serum MMP-7 and ADAM17 in different subgroups of CTD-ILD and lung function groups were analyzed,and the correlation between MMP-7 and ADAM17 with various indicators was elucidated,as well as their value in diagnosing CTD-ILD.Results:①Compared with the CTD group and the control group,the serum MMP-7 and ADAM17 levels in the CTD-ILD group were significantly increased(P<0.01);②In different subgroups of CTD-ILD,compared with the SS-ILD group,serum MMP-7 and ADAM17 levels were higher in the SSc-ILD group(P<0.05);③In different lung function groups of CTD-ILD,serum MMP-7 levels were as follows:the severe was greater than the moderate,and the moderate was greater than the mild(P<0.01),and serum ADAM17 levels were higher in the moderate and severe groups than in the mild group(P<0.01);④Correlation analysis showed that MMP-7 was positively correlated with CT score,CPI,and age in CTD-ILD patients(P<0.05),ADAM17 was positively correlated with CT score and CPI(P<0.01),and MMP-7 and ADAM17 were negatively correlated with FEV1,FVC,DLCO(%pred),and P(O_(2))(P<0.05).Conclusion:Se

关 键 词：结缔组织病 间质性肺病 基质金属蛋白酶-7 解整合素金属蛋白酶17 一氧化碳弥散量占预计值的百分比 复合生理指数 相关性 

分 类 号：R593.2[医药卫生—内科学] R563[医药卫生—临床医学]