洋金花体外抑制角质形成细胞炎性因子分泌和血管形成  

Datura metel L.inhibits the secretion of inflammatory factors and angiogenesis in keratinocytes in vitro

作　　者：仉博平 司昕蕾 吴芬芳 ZHANG Boping;SI Xinlei;WU Fenfang(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700;Central Laboratory,Shenzhen Hospital(Longgang),Beijing University of Chinese Medicine,Shenzhen 518172,China)

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京中医药大学深圳医院(龙岗)中心实验室,广东深圳518172

出　　处：《基础医学与临床》2024年第6期816-820,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(82172107);深圳市医学研究专项资金(SMRF.D2301015);深圳市龙岗区医疗卫生技术攻关重点项目(LGKCYLWS2022007)。

摘　　要：目的以肿瘤坏死因子(TNF-α)诱导角质形成细胞炎症为模型,研究传统草药洋金花(Datura metel L.)在银屑病治疗中可能的作用。方法使用角质形成细胞系HaCaT,构建了TNF-α诱导的银屑病细胞模型。实验分为3组:对照组、TNF-α银屑病模型组和TNF-α+洋金花干预组。以ELISA法测定IL-17和CCL20水平,利用Western blot检测核因子κB(NF-κB)亚单位p65蛋白的表达,使用体外血管生成分析试剂盒进行内皮细胞成血管实验。结果与TNF-α银屑病模型组相比,洋金花提取物极显著降低了TNF-α诱导的银屑病模型细胞培养上清液中的IL-17和CCL20的水平(P<0.001);洋金花提取物显著降低了TNF-α诱导的银屑病模型细胞的NF-κB亚单位p65的表达(P<0.01);洋金花提取物有效抑制了银屑病模型组细胞培养上清液对内皮细胞血管形成的诱导作用。结论洋金花提取物能够下调NF-κB信号通路分子,减少银屑病角质形成细胞炎性因子IL-17和CCL20的产生,抑制了血管形成。这些发现为洋金花在银屑病治疗中的应用提供了新的依据。Objective To investigate the potential role of the traditional herbal medicine Datura metel L.in the treatment of psoriasis using TNF-α-induced inflammation in keratinocytes as a model.Methods Keratinocyte cell line HaCaT was used to establish a psoriasis cell model by tumor necrosis factor-alpha(TNF-α)treatment.The experiment comprised three groups:a blank control group,TNF-α-induced psoriasis model group,and TNF-α+Datura metel L.intervention group.Level of IL-17 and CCL20 was measured ELISA,expression of nuclear factor kappa B(NF-κB)subunit p65 protein was measured by Western blot.Endothelial cell tube formation experiment was conducted using an in vitro angiogenesis analysis kit.Results Compared to the TNF-α-induced psoriasis model group,the Datura metel L.extract significantly reduced the levels of IL-17 and CCL20 in the cell culture supernatant of TNF-α-induced psoriasis model(P<0.001);Datura metel L.extract markedly decreased the NF-κB subunit p65 protein level in TNF-α-induced psoriasis model cells(P<0.01);Datura metel L.extract effectively inhibited the induction of endothelial cell tube formation by the cell culture supernatant of the psoriasis model group.Conclusions The Datura metel L.extract down-regulates NF-κB signaling pathway molecules,reducing the production of IL-17 and CCL20 inflammatory factors in inflammatory keratinocytes and inhibiting angiogenesis.

关 键 词：银屑病 洋金花 CCL20 血管形成 

分 类 号：R751.05[医药卫生—皮肤病学与性病学]