重金属汞胁迫下菲律宾蛤仔GPx和GST基因表达分析  

Expression Analysis of GPx and GST Genes in Ruditapes philippinarum Under Heavy Metal Mercury Stress

作　　者：郑志龙 闫路路 闫喜武 秦艳杰 ZHENG Zhi-long;YAN Lu-lu;YAN Xi-wu(College of Fisheries and Life Science,Dalian Ocean University,Dalian,Liaoning 116023;Liaoning Shellfish Breeding Engineering Technology Center,Dalian,Liaoning 116023)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023 [2]辽宁省贝类良种繁育工程技术中心,辽宁大连116023

出　　处：《安徽农业科学》2024年第10期113-119,共7页Journal of Anhui Agricultural Sciences

基　　金：山东省重点研发计划项目(2023LZGCQY001);现代农业产业技术体系专项(CARS-49);国家重点研发计划项目(2018YFD0901400)。

摘　　要：为研究重金属汞胁迫下菲律宾蛤仔谷胱甘肽过氧化物酶(glutathione peroxidase,GPx)和谷胱甘肽-S-转移酶(glutathione-S-transferase,GST)基因的表达情况,用汞对指示生物菲律宾蛤仔(Ruditapes philippinarum)进行单一慢性暴露试验,分别在0、12、24 h以及2、3、4、5、6、7和8 d时检测菲律宾蛤仔内脏团和鳃中GPx和GST基因的表达情况。结果表明,菲律宾蛤仔内脏团中GPx和GST基因的表达量都呈现波动变化趋势,分别在24和12 h时表达量最高(P<0.05),6 d时GPx基因表达量最低(P>0.05),3 d时GST基因表达量最低(P<0.05);GPx基因在鳃中的表达量在8 d时最高(P<0.05);鳃中GST基因的表达量在5 d时最高(P<0.05)。以上结果表明汞暴露在短期内能够诱导GPx和GST基因进行不同程度的表达,但其随着时间的延长呈明显的抑制作用。该研究结果为揭示重金属汞对菲律宾蛤仔的毒性机理提供了理论依据。In order to study the gene expression of glutathione peroxidase(GPx)and glutathione-S-transferase(GST)in Ruditapes philippinarum under the stress of heavy metal mercury(Hg),a single chronic exposure experiment on the indicator organism R.philippinarum was carried out.The expression of two genes in visceral mass and gill of R.philippinarum was detected at 0,12 h,24 h and 2,3,4,5,6,7 and 8 d.The results showed that the expression multiples of GPx and GST in the visceral mass showed a fluctuation trend,and the expression multiples reached the highest(P<0.05)at 24 and 12 h,respectively.The expression level was the lowest at 6 d(P>0.05)and 3 d(P<0.05).The expression multiple of GPx in the gill was the highest at 8 d(P<0.05),and the first peak appeared at 5 d.The expression amount of GST was the highest at 5 d(P<0.05).It showed that mercury exposure could induce the expression of GPx and GST in different degrees in the short term,but it had obvious inhibitory effect with the extension of time.This study provided the theoretical basis for revealing the toxicity of heavy metal mercury to R.philippinarum.

关 键 词：重金属汞 菲律宾蛤仔 谷胱甘肽过氧化物酶 谷胱甘肽-S-转移酶 

分 类 号：S917.4[农业科学—水产科学]