仙客来枯萎病病原菌产孢培养基筛选及培养条件优化  

Optimization of Sporulation Medium and Culture Conditions of the Pathogen of Cyclamen Wilt Disease

作　　者：宋卓琴 贾民隆 梁峥 梁晓霞 屈璐 王玉川 段九菊 曹冬梅 SONG Zhuo-qin;JIA Min-long;LIANG Zheng(College of Horticulture,Shanxi Agricultural University,Taiyuan,Shanxi 030031)

机构地区：[1]山西农业大学园艺学院,山西太原030031 [2]太原学院,山西太原030006 [3]长治市乐彩生物技术有限公司,山西长子046600

出　　处：《安徽农业科学》2024年第10期159-163,共5页Journal of Anhui Agricultural Sciences

基　　金：山西省农业科学院优秀青年基金项目(YCX2020YQ11)。

摘　　要：为了获得高浓度的仙客来枯萎病病原菌的孢子悬液,通过对不同营养培养基进行初筛,再以初筛营养成分进行正交试验,得到最佳营养配比,进一步通过单因素试验对培养基的pH和培养温度进行筛选。结果表明,马铃薯、绿豆、蔗糖为仙客来枯萎病病原菌产孢培养基的最佳营养成分,最佳配比为绿豆5.0%、蔗糖0.5%、马铃薯20%,培养基最佳pH为6,最佳培养温度为30℃。研究结果明确了仙客来枯萎病病原菌的产孢条件,为仙客来枯萎病的感病机理、防治技术及孢子萌发抑制等研究奠定基础,同时对指导实际生产中科学管理、综合防治病害具有重要意义。In order to obtain a high concentration spore suspension of the pathogen causing Cyclamen wilt disease,different nutrient media were preliminarily screened,and then orthogonal experiments were conducted to obtain the optimal nutrient ratio based on the initial screened nutrients.Furthermore,single factor experiments were conducted to screen the pH and culture temperature of the media.The results showed that potatoes,mung beans,and sucrose were the optimal nutritional components for the sporulation culture medium of the pathogen of Cyclamen wilt disease.The optimal ratio was mung bean 5.0%,sucrose 0.5%,and potato 20%.The optimal pH of the culture medium was 6,and the optimal culture temperature was 30℃.Clarifying the spore production conditions of the pathogen causing Cyclamen wilt disease lays the foundation for the research on the pathogenic mechanism,prevention and control techniques,and spore germination inhibition of Cyclamen wilt disease.At the same time,it is of great significance for guiding scientific management and comprehensive prevention and control of diseases in actual production.

关 键 词：仙客来枯萎病 病原菌 产孢培养基 培养条件 

分 类 号：S682.2[农业科学—观赏园艺]