拐枣汁加工工艺及二氢杨梅素含量测定方法研究  

Study on the Processing Technology of Hovenia dulcis Juice and Determination Method of Dihydromyricetin Content

作　　者：李晓杰 麻纪斌 舒泉湧 翟帆 李宗霖 LI Xiao-jie;MA Ji-bin;SHU Quan-yong(Shaanxi Pharmaceutical Holding Pharmaceutical Research Institute Co.,Ltd.,Xi’an,Shaanxi 710075;Shaanxi Institute of Traditional Chinese Medicine(Shaanxi Pharmaceutical Information Center),Xianyang,Shaanxi 712000)

机构地区：[1]陕西医药控股医药研究院有限公司,陕西西安710075 [2]陕西中药研究所(陕西医药信息中心),陕西咸阳712000

出　　处：《安徽农业科学》2024年第10期168-171,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究拐枣汁的加工工艺及果汁中二氢杨梅素的含量测定方法。[方法]采用单因素试验筛选拐枣汁的加工工艺,对比煎煮法及浸提法,考察粉碎粒度、加水量、浸泡时间,建立果汁中二氢杨梅素的含量测定方法并进行方法学考察。[结果]浸提法所得果汁口感好,拐枣粉粒度为过8目筛,加水量为1.3~1.5倍,浸泡时间为18 h。二氢杨梅素含量测定方法:色谱柱为Ajilent HC-C 18色谱柱,流动相为0.1%磷酸水-乙腈=18∶82,流速1 mL/min,检测波长290 nm,柱温30℃,进样量10μL。[结论]果汁加工工艺简单易行,口感较好,易于实现工业化生产。建立的二氢杨梅素含量测定方法准确度、精密度高,专属性好,为拐枣汁质量标准的制定提供了依据。[Objective]To study the processing technology of Hovenia dulcis juice and the determination method of dihydromyricetin content in the juice.[Method]A single factor experiment was used to screen the processing technology of Hovenia dulcis juice,and the decoction method and extraction method were compared.The crushing particle size,water addition amount and soaking time were examined.The determination method of dihydromyricetin content in Hovenia dulcis juice was established and methodological studies were conducted.[Result]The Hovenia dulcis juice obtained by the extraction method had a good taste,with the crushing particle size of 8 mesh sieves,the water addition amount of 1.3-1.5 times and the soaking time of 18 hours.The determination method for dihydromyricetin content was as follows:chromatographic column was Ajilent HC-C 18,mobile phase was 0.1%phosphoric acid solution∶acetonitrile=18∶82,flow rate was 1 mL/min,detection wavelength was 290 nm,column temperature was 30℃,injection amount was 10μL.[Conclusion]The juice processing technology is simple and feasible,the juice has a good taste and is easy to achieve industrial production.The determination method of dihydromyricetin content has high accuracy,precision and specificity,this provides a basis for the formulation of quality standards for Hovenia dulcis juice.

关 键 词：拐枣汁 二氢杨梅素 加工工艺 含量测定 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]