高效液相色谱法同时测定清热感冒颗粒中的6种黄酮类成分  

Simultaneous Determination of 6 Flavonoids in QingRe GanMao Granules by HPLC

作　　者：林丽清 LIN Li-qing(Xiamen Traditional Chinese Medicine Co.Ltd.Xiamen 361100,China)

机构地区：[1]厦门中药厂有限公司,福建厦门361100

出　　处：《海峡药学》2024年第4期42-44,共3页Strait Pharmaceutical Journal

摘　　要：目的 建立高效液相色谱法同时检测清热感冒颗粒中6种黄酮类成分的含量。方法 采用Agilent TC-C_(18)(4.6 mm×250 mm,5μm)色谱柱;以0.4%磷酸水溶液(A)-乙腈(B)为流动相,梯度洗脱;流速为1.0 mL·min^(-1),检测波长为360 nm,柱温为30℃。结果 6种黄酮类成分在各自相应浓度范围内与峰面积呈现良好的线性关系(r≥0.9997),加样回收率为95.4%~101.1%之间,相对标准偏差(RSD)≤3.01%。结论 该方法操作简单、准确可靠,可用于清热感冒颗粒中6种黄酮类成分的含量测定,并为清热感冒颗粒质量评价提供参考。OBJECTIVE In this study,a simple method was established for simultaneous determination of 6 flavonoids including rutin,luteolin,quercetin,apigenin,kaempferol and isorhamnetin in the QingRe GanMao Granules by HPLC.METHODS The analysis was performed on a 30 ℃ thermostatic Agilent TC-C_(18)(4.6 mm×250 mm,5 μm),with the mobile phase comprising of 0.4% phosphoric acid aqueous solution(A) and acetonitrile(B).The flow rate was 1.0 mL·min~(-1) and the detection wavelength was 360nm.RESULTS The curves of 6 target components showed a good linearity within their own ranges(r≥0.9997).In addition,the method recoveries were 95.4%-101.1%(RSD≤3.01%).CONCLUSION The method is simple,accurate,and convenient for the quality control of QingRe GanMao Granules.

关 键 词：清热感冒颗粒 高效液相色谱法 黄酮类成分 

分 类 号：R927[医药卫生—药学]