葛根素浓度对大鼠成骨细胞增殖的影响  

Effect of different puerarin concentrations on the proliferation of rat osteoblasts in vitro

作　　者：王海珍 周婷婷 徐波 Wang Haizhen;Zhou Tingting;Xu Bo(Hebei Rehabilitation Hospital,Qinhuangdao 066000,China)

机构地区：[1]河北省康复医院,河北省秦皇岛市066000

出　　处：《现代养生》2023年第17期1281-1284,共4页Health Protection and Promotion

摘　　要：目的 通过动物实验观察葛根素在体外对大鼠成骨细胞增殖能力的影响,探讨其临床作用机理。方法分离新生SD大鼠颅盖骨成骨细胞(OB),将分离培养后的OB随机分为7份,其中6份作为实验组分别放入不同浓度(1×10^(-5)mol/L~1×10^(-10)mol/L)的葛根素培养基中;1份作为对照组放入不含葛根素的培养基中。观察两组成骨细胞放入7种不同培养基后不同时间点的细胞形态、贴壁、生长、吸光度(OD值)等情况。结果显微镜下可见,三代OB培养第7d,对照组中细胞形态呈现出多种不规整形状;而实验组中在葛根素浓度为1×10^(-10)mol/L中细胞形态呈现出短梭形、三角形、多角形等,胞浆向外伸展;在葛根素浓度为1×10^(-7)mol/L中细胞形态单一,呈长梭形,胞浆向外伸展。增殖柱状图显示,培养3d后各组OB数量开始迅速增多,尤其是葛根素浓度达到1×10^(-7)mol/L组,细胞增殖数量远超过对照组和高浓度组,但此后随葛根素浓度降低,细胞增殖数量有逐渐降低的趋势。3d后葛根素浓度为1×10^(-7)mol/L及以下组的OD均显著升高(P<0.05);而5d和7d时,葛根素浓度为1×10^(-5)mol/L和1×10^(-6)mol/L的实验组OD值低于对照组(P<0.05)。表明当葛根素浓度在1×10^(-10)mol/L至1×10^(-7)mol/L范围内对OB均有促增值作用,且随着浓度的增高作用增强,但浓度再高则有抑制增值作用。结论 葛根素具有明显促进大鼠成骨细胞增殖作用,其作用与葛根素的浓度有关,当葛根素的浓度为1×10^(-7)mol/L对大鼠成骨细胞的促增殖作用最强。Objective To observe the effect of puerarin on the proliferation ability of rat osteoblasts in vitro through animal experiments,and explore its clinical mechanism of action.Method To isolate osteoblasts from the skull cap of newborn SD rats,the isolated and cultured osteoblasts were randomly divided into 7 parts,of which 6 were used as experimental groups and placed in different concentrations(1×10^(-5) mol/ml~1×10^(-10)mol/ml)of puerarin medium;1serving as the control group was placed in a culture medium without puerarin.Observe the cell morphology,adhesion,growth,and absorbance(OD) of the two components of bone cells at different time points after being placed in 7 different culture media.Results Under the microscope,it can be seen that on the 7th day of third-generation OB culture,the cell morphology in the control group showed various irregular shapes;In the experimental group,the concentration of puerarin was 1×10^(-10) mol/L medium cells exhibit short spindle shaped,triangular,polygonal shapes,and the cytoplasm extends outward;When the concentration of puerarin is 1×10^(-7) mol/L,the cell morphology is single,showing a long spindle shape,and the cytoplasm extends outward.The proliferation bar chart shows that after 3 days of cultivation,the number of OBs in each group began to rapidly increase,especially when the concentration of puerarin reached1×10^(-7) mol/L,the number of cell proliferation was much higher than that of the control group and low concentration group.However,as the concentration of puerarin decreased,the number of cell proliferation gradually decreased.After3 days,the concentration of puerarin is 1×10^(-7) mol/L The OD of groups with concentrations of and below significantly increased(P<0.05);At 5d and 7d,the concentration of puerarin is 1×10^(-5) mol/L and 1×10^(-6)mol/L.The OD value of the experimental group with was lower than that of the control group(P<0.05).Indicates that when the concentration of puerarin is at 1×10^(-10) mol/L to 1×10^(-7)mol/L Within the range of,there is

关 键 词：葛根素 葛根异黄酮 成骨细胞增殖 骨修复 

分 类 号：R285.5[医药卫生—中药学]