血清miR-98-5p、LncRNA XIST在急性呼吸窘迫综合征患儿中与疾病严重程度、预后的关系  

Relationship of serum miR-98-5p and LncRNA XIST in children with acute respiratory distress syndrome with disease severity and prognosis

作　　者：李书秀[1] 杜志云[2] LI Shuxiu;DU Zhiyun(Pediatric ICU,The First Hospital of Qinhuangdao,Qinhuangdao Hebei 066000,China;Department of Neonatology,The First Hospital of Qinhuangdao,Qinhuangdao Hebei 066000,China)

机构地区：[1]河北省秦皇岛市第一医院儿科ICU,066000 [2]河北省秦皇岛市第一医院新生儿科,066000

出　　处：《蚌埠医学院学报》2024年第4期488-492,共5页Journal of Bengbu Medical College

基　　金：河北省秦皇岛市科学技术研究与发展计划(202101A128)。

摘　　要：目的:检测急性呼吸窘迫综合征(ARDS)患儿血清中微小RNA(miR)-98-5p、长链非编码RNA(LncRNA)X染色体失活特异性转录因子(XIST)表达情况,并探究二者与疾病严重程度、预后的关系。方法:收集86例ARDS患儿为研究对象(研究组),同期健康体检儿童90名设为对照(对照组),参考柏林(2012年)ARDS病情严重程度诊断标准中动脉血氧分压/吸入氧浓度将ARDS患儿分为轻度组、中度组、重度组;另根据ARDS患儿28 d生存状况分为存活组和死亡组。实时荧光定量PCR(RT-PCR)法检测血清miR-98-5p、LncRNA XIST水平,绘制受试者工作特性(ROC)曲线分析血清中miR-98-5p、LncRNA XIST水平对ARDS患儿预后不良的预测价值;Kaplan-Meier生存曲线分析血清miR-98-5p、LncRNA XIST水平与ARDS患儿预后的关系;Pearson分析ARDS患儿血清中miR-98-5p与LncRNA XIST的相关性。结果:与对照组相比,研究组血清中miR-98-5p水平降低(P<0.01),LncRNA XIST水平升高(P<0.01)。与轻度组相比,中度组、重度组血清中miR-98-5p水平降低(P<0.05),LncRNA XIST水平升高(P<0.05);与中度组相比,重度组血清中miR-98-5p水平降低(P<0.05),LncRNA XIST水平升高(P<0.05)。与存活组相比,死亡组血清中miR-98-5p水平降低(P<0.01),LncRNA XIST水平升高(P<0.01)。ROC曲线显示,血清中miR-98-5p、LncRNA XIST水平预测ARDS患儿预后不良的ROC曲线下面积分别为0.771、0.764,截断值分别为0.54、1.97,其敏感性分别为76.1%、86.9%,特异性分别为73.7%、52.6%;血清中miR-98-5p联合LncRNA XIST预测ARDS患儿预后不良的ROC曲线下面积为0.888,其敏感性为77.6%、特异性为94.7%。miR-98-5p高表达者存活率35.14%高于低表达者12.24%(P<0.05),LncRNA XIST高表达存活率12.73%低于低表达者38.71%(P<0.01)。Pearson分析发现ARDS患儿血清中miR-98-5p与LncRNA XIST呈负相关关系(r=-0.411,P<0.05)。Starbase3.0预测发现LncRNA XIST与miR-98-5p存在结合位点。结论:ARDS患儿血清中miR-98-5p水平降低、LncRNA XISTObjective:To detect the expression of microRNA(miR)-98-5p and long non-coding RNA(LncRNA)X inactive specific transcript(XIST)in serum of children with acute respiratory distress syndrome(ARDS),and to explore the relationship between miR-98-5p and LncRNA XIST and disease severity and prognosis.Methods:A total of 86 children with ARDS were selected as study sujects(study group).During the same period,90 healthy children were set as controls(control group).Children with ARDS were divided into mild,moderate and severe groups according to the arterial partial pressure of oxygen/inspired oxygen concentration in the Berlin(2012)diagnostic criteria for the severity of ARDS.In addition,children with ARDS were divided into the survival group and death group according to their 28-day survival status.Serum levels of miR-98-5p and LncRNA XIST were detected by real-time PCR(RT-PCR).Receiver operating characteristic(ROC)curves were drawn to analyze the predictive value of serum miR-98-5p and LncRNA XIST levels for the poor prognosis in children with ARDS.Kaplan-Meier survival curve was used to analyze the relationship between the serum levels of miR-98-5p and LncRNA XIST and the prognosis of children with ARDS;Pearson analysis was performed to analyze the correlation between miR-98-5p and LncRNA XIST in serum of children with ARDS.Results:Compared with the control group,the serum level of miR-98-5p in the study group decreased(P<0.01),and the level of LncRNA XIST increased(P<0.01).Compared with the mild group,the serum level of miR-98-5p in the moderate and severe groups decreased(P<0.05),and the level of LncRNA XIST increased(P<0.05);compared with the moderate group,the serum level of miR-98-5p in the severe group decreased(P<0.05),and the level of LncRNA XIST increased(P<0.05).Compared with the survival group,the serum level of miR-98-5p in the death group decreased(P<0.01),and the level of LncRNA XIST increased(P<0.01).The ROC curve showed that the area under the ROC curve of serum miR-98-5p and LncRNA XIST levels in predict

关 键 词：急性呼吸窘迫综合征 微小RNA-98-5p 长链非编码RNA X染色体失活特异性转录因子 疾病严重程度 预后 

分 类 号：R563.8[医药卫生—呼吸系统]