小麦耐盐基因TaHKT1的新单倍型及其耐盐性评价  

New Haplotype of Salt Tolerance Gene TaHKT1 and Evaluation of Salt Tolerance in Wheat

作　　者：钮力亚[1] 邹景伟 王伟伟[1] 王志 张玉杰 罗政辉 赵振杰 于亮[1] NIU Liya;ZOU Jingwei;WANG Weiwei;WANG Zhi;ZHANG Yujie;LUO Zhenghui;ZHAO Zhenjie;YU Liang(Cangzhou Academy of Agriculture and Forestry Sciences,Cangzhou Hebei 061001,China)

机构地区：[1]沧州市农林科学院,河北沧州061001

出　　处：《种子》2024年第4期51-63,共13页Seed

基　　金：河北省耐盐碱作物种业创新团队(23327501D);河北省重点研发计划项目(现代种业科技创新专项)“小麦现代种业科技创新团队”(21326318D);财政部和农业农村部现代农业产业技术体系专项(CARS-03)。

摘　　要：本研究利用0.5%盐胁迫对275份小麦品种(系)进行芽期耐盐性鉴定,测定发芽率、芽长、根长、根数、芽鲜质量、根鲜质量及根冠比等指标。利用主成分分析法将7个指标转化为3个主成分,累积贡献率为74.35%。通过主成分贡献率和隶属函数分析进一步将3个主成分简化为综合评价值(D)。根据D值的大小,利用聚类分析法将275份小麦品种(系)划分为高耐盐、中耐盐、耐盐、盐敏感和盐高敏感共5个等级,筛选出高耐盐种质11份、中耐盐107份、耐盐60份,累计占比64.7%。通过对13个小麦品种(系)进行TaHKT1基因序列分析,发现T∶T新单倍型与耐盐性相关。利用KASP标记对275份小麦品种(系)进行检测,有98份小麦品种(系)为新单倍型,占35.64%;新单倍型小麦品种(系)在各省份中所占比例不同。将小麦品种(系)的TaHKT1基因T∶T单倍型与耐盐性D值进行关联分析,结果表明,TaHKT1基因T∶T单倍型与小麦芽期耐盐性差异达极显著水平(p<0.01)。In this study,under the 0.5%salt stress treatment,275 wheat varieties(lines)were identified for salt tolerance during the germination stage,and relative indicators such as germination rate,shoot length,root length,root number,shoot fresh weight,root fresh weight,and root crown ratio were measured.The salt tolerance of 7 indicators was simplified into 3 principal components using principal component analysis,with a cumulative contribution rate of 74.35%.Through the analysis of principal component contribution rate and membership function,the 3 principal component values were further reduced to D-value of comprehensive evaluation index.According to D-value and cluster analysis,275 wheat varieties(lines)were divided into 5 classes of salt resistance,including high salt tolerance,salt tolerance,medium salt tolerance,salt sensitivity,and high salt sensitivity.Eleven,one hundred and seven,and sixty wheat varieties(lines)with high salt tolerance,medium salt tolerance,and salt tolerance above were selected,accounting for 64.7%of the total.The TaHKT1 gene sequence analysis of 13 wheat varieties(lines)revealed that the new T∶T haplotype was related to salt tolerance.275 wheat varieties(lines)were tested by KASP markers,98 of which were the new haplotype,accounting for 35.64%.The proportion of new haplotype wheat varieties(lines)in different provinces were different.The correlation analysis between the T∶T genotype of wheat varieties(lines)and the salt tolerance D-value showed that the new haplotype of TaHKT1 gene was significantly different from the salt tolerance of wheat at the bud stage(p<0.01).

关 键 词：小麦 耐盐性 TaHKT1基因 新单倍型 

分 类 号：S512.1[农业科学—作物学]