黑木耳UGP基因家族全基因组鉴定及其与菌丝生长多糖合成的相关性研究  

Genome-Wide Identification of the UGP Gene Family in Auricularia heimuer and Its Correlation with Mycelial Growth and Polysaccharide Synthesis

作　　者：苏世贤 吴媛 刘翀 江苏燕 刘青媛 陈孟谈 龙茶 汪淘 徐彦军[1,2] 田风华 韦金江 郎云龙 牟海林 徐乾城 李伟[1,2,3] SU Shixian;WU Yuan;LIU Chong;JIANG Suyan;LIU Qingyuan;CHEN Mengtan;LONG Cha;WANG Tao;XU Yanjun;TIAN Fenghua;WEI Jinjiang;LANG Yunlong;MOU Hailin;XU Qiancheng;LI Wei(College of Agriculture,Guizhou University,Guiyang 550025,China;Institute of Edible Fungi,Guizhou University,Guiyang 550025,China;Engineering Research Center for Protected Vegetable Crops in Institutions of Higher Learning of Guizhou Province,Guiyang 550025,China;Guizhou Plateau Lanmeng Mushroom Technology Co.,Ltd.,Bijie Guizhou 551700,China)

机构地区：[1]贵州大学农学院,贵阳550025 [2]贵州大学食用菌研究院,贵阳550025 [3]贵州省高等学校设施蔬菜工程研究中心,贵阳550025 [4]贵州高原蓝梦菇业科技有限公司,贵州毕节551700

出　　处：《种子》2024年第4期69-80,共12页Seed

基　　金：贵州省高等学校设施蔬菜工程研究中心平台建设项目(黔教技[2022]040号);贵州省科技重大专项(黔科合重大专项字[2019]3005);贵州省教育厅食用菌产业导师服务乡村振兴产业发展示范基地(黔教技[2022]071号);贵州大学大学生创新创业训练计划项目(gzuxc2023085);贵州省农业农村厅食用菌产业项目(黔财农2019[143]号);贵州省教育厅农业重大产业攻关项目(黔教合KY字[2019]017)。

摘　　要：黑木耳多糖是具有药用价值的生物活性分子,然而黑木耳高多糖的合成分子机理尚未明确。本研究对处于多糖合成途径关键位置的UGP家族基因进行了鉴定,从黑木耳基因组中鉴定出2个AhUGPs基因,并从另外21个食用菌基因组内挖掘出39个UGPs基因,通过生物信息学分析发现,UGP基因家族成员在真菌体内具有较高的保守性,成员数量少但仍发挥着重要作用,木耳属的4个UGPs则表现出了更高的保守性。在对黑木耳AhUGPs的启动子区域分析中发现了较多的光响应元件和激素响应元件,说明AhUGPs较易受到光和激素调节。在对AhUGPs的表征中,发现AhUGP1的表达水平与菌丝生长速度、菌丝生物量、多糖含量都显著相关,相较于AhUGP2,AhUGP1在菌丝生长和多糖合成中的重要性更高。The polysaccharides in Auricularia heimuer are bioactive molecules with medicinal value.However,the molecular mechanism of the synthesis of polysaccharides in A.heimuer has not yet been clarified.This study identified UGP family genes at key positions in the polysaccharide synthesis pathway.Two AhUGPs genes were identified from the A.heimuer genome,and 39 UGPs genes were unearthed from another 21 edible fungus genomes through bioinformatics analysis.It was found that the members of the UGP gene family were highly conserved in fungi.The number of members was small but they still played an important role.The four UGPs of the genus Auricularia showed higher conservation.In the analysis of the promoter region of AhUGPs,more light-responsive elements and phytohormone-responsive elements were found,indicating that AhUGPs were more susceptible to light and hormone regulation.In the characterization of AhUGPs,it was found that the expression level of AhUGP1 was significantly related to mycelial growth rate,mycelial biomass,and polysaccharide content.Compared with AhUGP2,AhUGP1 was more important in hyphal growth and polysaccharide synthesis.

关 键 词：黑木耳 UGP基因家族 多糖合成 菌丝生长 

分 类 号：S646.6[农业科学—蔬菜学]