复合盐碱胁迫对沙葱种子萌发的影响  

Effects of Compound Saline-alkali Stress on Seed Germination of Allium mongolicum Regel

作　　者：曹镇宇 魏鹏超 杨忠仁[1] 张凤兰[1] CAO Zhenyu;WEI Pengchao;YANG Zhongren;ZHANG Fenglan(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Key Laboratory of Germplasm Resources and Germplasm Innovation of Wild Endemic Vegetables of Inner Mongolia,Hohhot 010019,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,内蒙古自治区野生特有蔬菜种质资源与种质创新重点实验室,呼和浩特010019

出　　处：《种子》2024年第4期104-110,116,共8页Seed

基　　金：国家自然科学基金项目(31760081);内蒙古自然科学基金项目(2019MS03055);内蒙古自治区种业科技创新重大示范工程揭榜挂帅项目;内蒙古自治区科技重大专项(2019ZD016);内蒙古自治区应用技术研究与开发项目(2019GC237)。

摘　　要：以沙葱种子为试验材料,以盐分种类和盐浓度为变量,将4种盐(NaCl、Na_(2)SO_(4)、NaHCO_(3)、Na_(2)CO_(3))按摩尔比以A(1∶1∶0∶0)、B(1∶2∶1∶0)、C(1∶9∶9∶1)、D(1∶1∶1∶1)、E(9∶1∶1∶9)5种不同配比进行混合,共5个组别,每个组分别设置8个浓度梯度进行纸上萌发试验。结果表明,不同盐分组合中的各浓度处理下沙葱种子的萌发均受到一定程度的抑制。胁迫浓度升高,发芽率、发芽势、活力指数、发芽指数下降,相对盐害率逐渐升高,种子萌发受到的抑制作用增强。盐分浓度对种子萌发的影响大于盐分种类,沙葱对中性盐胁迫的耐受性强于碱性盐。通过回归分析与聚类分析,得出沙葱的耐盐阈值为23.553 mmol/L,耐盐极限值为236.087 mmol/L。通过主成分分析,将萌发指标转换为两个主成分,累积贡献率为93.861%,能够较为有效地对实验数据包含的信息进行反映,构建综合得分模型:C=0.913×C_(1)+0.087×C_(2)。结合回归分析、聚类分析与主成分得分模型建立沙葱综合评价体系,对沙葱耐盐碱品种选育提供有效手段。The seeds of Allium mongolicum Regel were used as experimental materials,and the types and concentrations of salt were used as variables.The four salts(NaCl,Na_(2)SO_(4),NaHCO_(3),Na_(2)CO_(3))were mixed according to the molar ratio in 5 different ratios:A(1∶1∶0∶0),B(1∶2∶1∶1∶0),C(1∶9∶9∶1),D(1∶1∶1∶1),E(9∶1∶1∶1∶9),and a total of 5 groups were set up with 8 concentration gradients for each group and the paper germination test was conducted.The results showed that the germination of Allium mongolicum Regel seeds was inhibited to a certain extent under different salt combinations and different concentration treatments.With the increase of stress concentration,germination rate,germination potential,vitality index and germination index decreased,the relative salt damage rate gradually increased,and the inhibitory effect on seed germination was enhanced.The effect of salt concentration on seed germination was greater than that of salt type.The tolerance to neutral salt stress was stronger than alkaline salt stress.By regression analysis and cluster analysis,the salt tolerance threshold was 23.553 mmol/L,and the salt tolerance limit was 236.087 mmol/L.By principal component analysis,the germination index was converted into two principal components,and the cumulative contribution rate was 93.861%,which could effectively reflect the information contained in the experimental data and build a comprehensive score model:C=0.913×C_(1)+0.087×C_(2).Combined with regression analysis,cluster analysis and principal component score model,a comprehensive evaluation system was established to provide an effective means for saline-alkali tolerant breeding of Allium mongolicum Regel.

关 键 词：种子萌发 沙葱 盐碱胁迫 主成分分析 

分 类 号：S633.9[农业科学—蔬菜学]