3种病害海带的颜色差异及生化组分分析  

Analysis of Appearance Color and Biochemical Composition in Three Types of Diseased Kelp Laminaria japonica

作　　者：刘晓慧 刘玮 王翔宇 吕芳 吴海一 詹冬梅 LIU Xiaohui;LIU Wei;WANG Xiangyu;LYU Fang;WU Haiyi;ZHAN Dongmei(Qingdao Key Laboratory of Coastal Ecological Restoration and Security,Qingdao Macroalgae Engineering Technology Research Center,Shandong Province Macroalgae Resources Conservation and Application Engineering Research Center,Marine Science Research Institute of Shandong Province,Qingdao 266104,China)

机构地区：[1]山东省海洋科学研究院,山东省大型海藻资源保护与应用工程技术研究中心,青岛市大型海藻工程技术研究中心,青岛市近海生态修复与安全保障重点实验室,山东青岛266104

出　　处：《水产科学》2024年第3期372-380,共9页Fisheries Science

基　　金：山东省现代农业藻类产业技术体系项目(SDAIT-26);山东省农业良种工程项目(2021LZGC004).

摘　　要：为分析不同病害海带叶片颜色和生化组分的差异及二者间的关系,分别对患点状白烂病、绿烂病和卷曲病3种病害海带的颜色指标(明度、红绿色度、黄蓝色度、褐度、色泽饱和度和色调角)和生化组分含量(含水率、叶绿素a、类胡萝卜素、可溶性蛋白和褐藻酸盐)进行了差异分析及相关性分析探索。结果表明:3种病害海带的总色差值ΔE为7.41~15.24,明度、红绿色度、黄蓝色度和色泽饱和度等颜色指标差异显著(P<0.05),其中红绿色度的变异系数最高,为48.18%。绿烂病海带红绿色度最低,明度、黄蓝色度和色泽饱和度最高;卷曲病海带与之相反,红绿色度最高,明度、黄蓝色度和色泽饱和度最低;点状白烂病海带各颜色指标介于二者之间。3种病害海带的叶绿素a、类胡萝卜素和可溶性蛋白含量差异显著(P<0.05),其中可溶性蛋白含量的变异系数最高,为22.08%。点状白烂病海带的叶绿素a含量最高,可溶性蛋白含量最低,绿烂病海带的类胡萝卜素含量最低,卷曲病海带的类胡萝卜素含量最高。3种病害海带基于颜色指标和生化组分含量的综合判别率分别为78.90%和100.00%。绿烂病海带的红绿色度与叶绿素a、类胡萝卜素含量呈显著正相关(r=0.99,r=0.99),3种病害海带的明度、黄蓝色度和色泽饱和度等颜色指标与可溶性蛋白含量存在较强的相关关系(|r|=0.94~0.99)。In order to understand the variances and relationships between appearance color and biochemical composition of different diseased kelp Laminaria japonica,the differences in color indices(L*,a*,b*,BI,c*,and h*)and biochemical composition contents(moisture,chlorophyll a,carotenoid,soluble protein,alginate)were compared in three types of diseased kelp(spot white rotten kelp,green rotten kelp and twisted kelp)sampled from Rongcheng mariculture region by variance analysis and discriminant analysis.Correlation analysis between color indices and biochemical composition contents was also conducted for further exploration.The results showed that the chromatism value was ranged from 7.41 to 15.24 in the three types of diseased kelp and the color indices of L*,a*,b*and c*were significantly different(P<0.05),in which the variable coefficient of a*was the maximal with 48.18%.The green rotten kelp had the minimal a*,the maximal L*,b*,c*values,and the contrast case was observed in the twist kelp.The color indices value of spot white rotten kelp was between the green rotten kelp and twisted kelp.The biochemical components of chlorophyll a,carotenoid and soluble protein were significantly different(P<0.05)in the three types of diseased kelp,with the maximal variable coefficient of soluble protein content(22.08%).There were the maximal content of chlorophyll a and the minimal content of soluble protein in spot white rotten kelp and green rotten kelp had the minimal content of carotenoid and twist kelp the maximal content of carotenoid.The total discrimination accuracy was found to be 78.90%based on color indices and 100.00%based on biochemical composition contents in the three types of diseased kelp.The color index a*was found to be positive correlated with the contents of chlorophyll a and carotenoid in green rotten kelp samples(r=0.99,r=0.99),and there existed strong correlation between color indices of L*,b*,c*and the content of soluble protein in all the three types of diseased kelp(|r|=0.94—0.99).

关 键 词：点状白烂病 绿烂病 卷曲病 海带 颜色 生化组分 

分 类 号：S968.421[农业科学—水产养殖]