大连地区养殖日本囊对虾5种病原携带情况调查  

Detection of Five Pathogens from Kuruma Prawn Marsupenaeus japonicus Farmed in Dalian Region

作　　者：陈文博 任雪 李娜 张赛赛 李林晗 曾范双 韩雨哲 任同军 CHEN Wenbo;REN Xue;LI Na;ZHANG Saisai;LI Linhan;ZENG Fanshuang;HAN Yuzhe;REN Tongjun(Dalian Ocean Development Affairs Service Center,Dalian 116023,China;College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;Dalian Supply and Marketing Asset Operation Co.,Ltd,Dalian 116023,China)

机构地区：[1]大连市海洋发展事务服务中心,辽宁大连116023 [2]大连海洋大学水产与生命学院,辽宁大连116023 [3]大连供销资产运营有限公司,辽宁大连116023

出　　处：《水产科学》2024年第3期410-419,共10页Fisheries Science

基　　金：大连市财政支农〔2020〕4号项目;大连市农业农村局水产养殖优势品种病害及养殖环境风险预警预报项目(大农发〔2020〕11号).

摘　　要：为探明大连主养区日本囊对虾血细胞虹彩病毒、白斑综合征病毒、传染性皮下及造血组织坏死病毒、急性肝胰腺坏死病致病性副溶血弧菌和肝肠胞虫的携带情况,对2019年9月采集自辽宁省大连市城子坦、青堆、仙浴湾等8个主养区的日本囊对虾样本进行了5种病原的PCR检测。结果显示:日本囊对虾携带的主要病原依次为白斑综合征病毒、血细胞虹彩病毒、肝肠胞虫、传染性皮下及造血组织坏死病毒和急性肝胰腺坏死病致病性副溶血弧菌,其阳性检出率分别为9.99%、4.30%、3.74%、2.24%和1.05%;此外,城子坦地区对虾的白斑综合征病毒和急性肝胰腺坏死病致病性副溶血弧菌携带率最高,青堆、仙浴湾和石河对虾的血细胞虹彩病毒、传染性皮下及造血组织坏死病毒和肝肠胞虫阳性率最高。本调查结果可为大连地区养殖日本囊对虾病原控制策略的制定提供参考依据。Shrimp hemocyte iridescent virus(SHIV),white spot syndrome virus(WSSV),infectious hypodermal and hematopoietic necrosis virus(IHHNV),Vibrio parahaemolyticus acute hepatopancreatic necrosis disease(VPAHPND),and Enterocytozoon hepatopenaei(EHP)were detected in samples of kuruma prawn Penaeus japonicus with body weight of(22.5±2.5)g collected from the main farm regions in Dalian region,Liaoning Province,including Chengzitan,Xianyuwan,Qingdui,and other 5 regions in September 2019 by PCR methods.The results showed that the main pathogens carried by the kuruma prawn were WSSV,SHIV,EHP,IHHNV and VPAHPND,with positive detection rates of 9.99%,4.30%,3.74%,2.24%,and 1.05%,respectively.In addition,WSSV and VPAHPND from Chengzitan region showed the maximal positive rates,and SHIV,IHHNV and EHP from Qingdui,Xianyuwan and Shihe regions showed the maximal positive rates.The findings will provide scientific reference for the pathogen control strategies for kuruma prawn culture in Dalian.

关 键 词：日本囊对虾 病原 巢式PCR 携带 

分 类 号：S968.22[农业科学—水产养殖]