Simultaneous dual-region two-photon imaging of biological dynamics spanning over 9 mm in vivo  

作　　者：CHI LIU CHENG JIN JUNHAO DENG JUNHAO LIANG LICHENG ZHANG LINGJIE KONG 

机构地区：[1]State Key Laboratory of Precision Measurement Technology and Instruments,Department of Precision Instrument,Tsinghua University,Beijing 100084,China [2]School of Life Sciences,Tsinghua University,Beijing 100084,China [3]Senior Department of Orthopedics,The Fourth Medical Center of Chinese PLA General Hospital,Beijing 100853,China [4]IDG/McGovern Institute for Brain Research,Tsinghua University,Beijing 100084,China

出　　处：《Photonics Research》2024年第3期456-464,共9页光子学研究（英文版）

基　　金：STI2030-Major Projects(2022ZD0212000);National Natural Science Foundation of China(32021002,61831014);"Bio-Brain+X”Advanced Imaging Instrument Development Seed Grant。

摘　　要：Biodynamical processes,especially in system biology,that occur far apart in space may be highly correlated.To study such biodynamics,simultaneous imaging over a large span at high spatio-temporal resolutions is highly desired.For example,large-scale recording of neural network activities over various brain regions is indispensable in neuroscience.However,limited by the field-of-view(FoV)of conventional microscopes,simultaneous recording of laterally distant regions at high spatio-temporal resolutions is highly challenging.Here,we propose to extend the distance of simultaneous recording regions with a custom micro-mirror unit,taking advantage of the long working distance of the objective and spatio-temporal multiplexing.We demonstrate simultaneous dual-region two-photon imaging,spanning as large as 9 mm,which is 4 times larger than the nominal FoV of the objective.We verify the system performance in in vivo imaging of neural activities and vascular dilations,simultaneously,at two regions in mouse brains as well as in spinal cords,respectively.The adoption of our proposed scheme will promote the study of systematic biology,such as system neuroscience and system immunology.

关 键 词：RECORDING VIVO DYNAMICS 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术] R318[自动化与计算机技术—计算机科学与技术]