Expansion and improvement of ChinaMu by MuT-seq and chromosome-level assembly of the Mu-starter genome  

作　　者：Lei Liang Yuancong Wang Yanbin Han Yicong Chen Mengfei Li Yibo Wu Zeyang Ma Han Zhao Rentao Song 

机构地区：[1]State Key Laboratory of Maize Bio-breeding,Frontiers Science Center for Molecular Design Breeding,Joint International Research Laboratory of Crop Molecular Breeding,National Maize Improvement Center,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100091,China [2]Institute of Crop Germplasm and Biotechnology,Jiangsu Provincial Key Laboratory of Agrobiology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China [3]Sanya Institute of China Agricultural University,Sanya 572025,China [4]Hainan Yazhou Bay Seed Laboratory,Sanya 572025,China

出　　处：《Journal of Integrative Plant Biology》2024年第4期645-659,共15页植物学报（英文版）

摘　　要：ChinaMu is the largest sequence-indexed Mutator(Mu)transposon insertional library in maize(Zea mays).In this study,we made significant improvements to the size and quality of the ChinaMu library.We developed a new Mu-tag isolation method Mu-Tn5-seq(MuT-seq).Compared to the previous method used by ChinaMu,MuT-seq recovered 1/3 more germinal insertions,while requiring only about 1/14 of the sequencing volume and 1/5 of the experimental time.Using MuT-seq,we identified 113,879 germinal insertions from 3,168 Mu-active F1 families.We also assembled a high-quality genome for the Mu-active line Mu-starter,which harbors the initial active MuDR element and was used as the pollen donor for the mutation population.Using the Mu-starter genome,we recovered 33,662(15.6%)additional germinal insertions in 3,244(7.4%)genes in the Mu-starter line.The Mu-starter genome also improved the assignment of 117,689(54.5%)germinal insertions.The newly upgraded ChinaMu dataset currently contains 215,889 high-quality germinal insertions.These insertions cover 32,224 pan-genes in the Mu-starter and B73Ref5 genomes,including 23,006(80.4%)core genes shared by the two genomes.As a test model,we investigated Mu insertions in the pentatricopeptide repeat(PPR)superfamily,discovering insertions for 92%(449/487)of PPR genes in ChinaMu,demonstrating the usefulness of ChinaMu as a functional genomics resource for maize.

关 键 词：ChinaMu Mutator MuT-seq PPR gene Zea mays 

分 类 号：S513[农业科学—作物学]