Structural insights into the Oryza sativa cation transporters HKTs in salt tolerance  

作　　者：Ran Gao Yutian Jia Xia Xu Peng Fu Jiaqi Zhou Guanghui Yang 

机构地区：[1]State Key Laboratory of Plant Environmental Resilience,Frontiers Science Center for Molecular Design Breeding,College of Biological Sciences,China Agricultural University,Beijing 100193,China

出　　处：《Journal of Integrative Plant Biology》2024年第4期700-708,共9页植物学报（英文版）

基　　金：supported by the National Key R&D Program of China(2022YFA1303400);National Natural Science Foundation of China(32171188 to G.Y.);Chinese Universities Scientific Fund funds(2023RC012,2023TC190);Young Elite Scientists Sponsorship Program by China Association for Science and Technology(to G.Y.);China Agriculture University Young Talent Program in Life Science(Grant No.008);Pinduoduo-China Agricultural University Research Fund(Grant No.PC2023B01010)。

摘　　要：The high-affinity potassium transporters(HKTs),selectively permeable to either Na^(+)alone or Na^(+)/K^(+),play pivotal roles in maintaining plant Na^(+)/K^(+)homeostasis.Although their involvement in salt tolerance is widely reported,the molecular underpinnings of Oryza sativa HKTs remain elusive.In this study,we elucidate the structures of OsHKT1;1 and OsHKT2;1,representing two distinct classes of rice HKTs.The dimeric assembled OsHKTs can be structurally divided into four domains.At the dimer interface,a half-helix or a loop in the third domain is coordinated by the C-terminal region of the opposite subunit.Additionally,we present the structures of OsHKT1;5 salt-tolerant and salt-sensitive variants,a key quantitative trait locus associated with salt tolerance.The salt-tolerant variant of OsHKT1;5 exhibits enhanced Na^(+)transport capability and displays a more flexible conformation.These findings shed light on the molecular basis of rice HKTs and provide insights into their role in salt tolerance.

关 键 词：HKT SALT-TOLERANT STRUCTURE 

分 类 号：S511[农业科学—作物学]