OsWRKY78 regulates panicle exsertion via gibberellin signaling pathway in rice  

作　　者：Enyang Mei Mingliang He Min Xu Jiaqi Tang Jiali Liu Yingxiang Liu Zhipeng Hong Xiufeng Li Zhenyu Wang Qingjie Guan Xiaojie Tian Qingyun Bu 

机构地区：[1]State Key Laboratory of Black Soils Conservation and Utilization,Key Laboratory of Soybean Molecular Design Breeding,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Northeast Forestry University,Ministry of Education,Harbin 150040,China [4]College of Life Science,Northeast Forestry University,Harbin 150040,China

出　　处：《Journal of Integrative Plant Biology》2024年第4期771-786,共16页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.31671653,31801017);Heilongjiang Key Research and Development Program(Grant No.2022ZX02B03);National Natural Science Foundation of China-Heilongjiang Joint Fund(Grant No.U23A20193);Youth Innovation Promotion Association Chinese Academy of Sciences(Grant No.2021229);Young Scientist Group Project of Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences(Grant No.2023QNXZ02)。

摘　　要：Panicle exsertion is one of the crucial agronomic traits in rice(Oryza sativa).Shortening of panicle exsertion often leads to panicle enclosure and severely reduces seed production.Gibberellin(GA)plays important roles in regulating panicle exsertion.However,the underlying mechanism and the relative regulatory network remain elusive.Here,we characterized the oswrky78 mutant showing severe panicle enclosure,and found that the defect of oswrky78 is caused by decreased bioactive GA contents.Biochemical analysis demonstrates that OsWRKY78 can directly activate GA biosynthesis and indirectly suppress GA metabolism.Moreover,we found OsWRKY78 can interact with and be phosphorylated by mitogen-activated protein kinase(MAPK)kinase OsMAPK6,and this phosphorylation can enhance OsWRKY78 stability and is necessary for its biological function.Taken together,these results not only reveal the critical function of OsWRKY78,but also reveal its mechanism via mediating crosstalk between MAPK and the GA signaling pathway in regulating panicle exsertion.

关 键 词：GIBBERELLIN MAPK cascades OsWRKY78 panicle exsertion RICE 

分 类 号：S511[农业科学—作物学]