浙江省晚粳稻主栽品种白叶枯病抗性鉴定及抗病基因检测  

Resistance Identification of Bacterial Blight and Resistance Gene Test of Commercial Cultivars of Japonica Rice in Zhejiang

作　　者：谢留杰[1] 段敏[1] 余山红[1] 潘晓飚 陈析丰[2] 黄善军 Xie Liujie;Duan Min;Yu Shanhong;Pan Xiaobiao;Chen Xifeng;Huang Shanjun(Taizhou Research Academy of Agriculture Sciences,Linhai,317000;College of Chemistry and Life Science,Zhejiang Normal University,Jinhua,321004)

机构地区：[1]台州市农业科学研究院,临海317000 [2]浙江师范大学化学与生命科学学院,金华321004

出　　处：《分子植物育种》2024年第10期3204-3212,共9页Molecular Plant Breeding

基　　金：台州市科技计划项目(21nya07);台州市农业类科研攻关项目(1901ny02);浙江省重大科研攻关项目(2016C-02050-5)共同资助。

摘　　要：对包括浙江省主栽品种在内的75份粳稻材料接种白叶枯病菌‘浙173’,在接种后不同时间观察发病情况,同时利用11个白叶枯病抗病基因连锁或功能性分子标记对接种后表现为抗病的材料进行基因型检测。结果表明:常规粳稻材料中‘ZH18-117’、‘春江糯6号’、和‘秀水63’等3个品种抗病性最佳,13份杂交粳稻仅‘嘉优中科2号’表现为抗病。被列入浙江省主导品种的常规粳稻‘浙粳99’、‘嘉67’、‘中嘉8号’表现为抗病,杂交粳稻仅‘甬优538’表现为中抗。不同品种动态发病研究显示:随着抗性的提高,水稻在后期抑制病斑的扩散能力也增强。白叶枯病抗性基因标记检测结果显示:‘嘉优中科2号’、‘浙粳60’和‘浙粳99’检测到与Xa1基因阳性对照‘三黄占2号’一致的条带;‘秀水09’、‘秀水63’、‘嘉花1号’、‘春江16A’等4个品种能扩增出Xa4基因抗性条带;其余抗病品种未检测到抗病基因。In this study,seventy-five japonica rice materials including the main cultivars of Zhejiang Province were inoculated with bacterial blight pathogen‘Zhe173’,and the resistance identification was analyzed at different times after inoculation.Meanwhile,11 resistance genes against bacterial blight,which have been reported were analyzed via functional or linkage molecular markers in resistance materials.The results showed that the conventional japonica rice varieties‘ZH18-117’,‘Chunjiangnuo6’and‘Xiushui63’displayed the best disease resistance.However,only‘Jiayouzhongke2’showed resistance during 13 hybrid japonica rice varieties.During the recommended cultivars of Zhejiang Province,conventional japonica rice‘Zhejing99’,‘Jia67’and‘Zhongjia8’were tested as resistance,and only‘Yongyou538’showed middle resistance in all hybrid japonica rice varieties.Furthermore,with the improvement of resistance,rice could inhibit the spread of disease spots much better during the mature period.Last but not least,molecular marker identification of 11 reported resistance genes has been studied.For Xa1,‘Jiayouzhongke2’,‘Zhejing60’and ‘Zhejing99’have the same band with positive control‘Sanhuangzhan2’.For Xa4,four rice varieties,‘Xiushui09’,‘Xiushui63’,‘Jiahua1’and‘Chunjiang16A’showed the resistance band.The rest resistance materials didn't have tested resistance genes.

关 键 词：粳稻 主栽品种 白叶枯病 抗性鉴定 分子标记 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]