基于ISSR标记的12份樱花种质亲缘关系分析  

Genetic Relationship Analysis of 12 Cerasus spp.Germplasms Based on SRAP and ISSR Marker

作　　者：刘丽丽 刘汝明 刘燕 李建辉 Liu Lili;Liu Ruming;Liu Yan;Li Jianhui(College of Chemistry and Materials Engineering,Quzhou University,Quzhou,324000;Quzhou Academy of Agricultural and Forestry Sciences,Quzhou,324000;Forestry Technology Extension Center,Kecheng District of Quzhou City,Quzhou,324000;Quzhou Wuxijiang National Wetland Park Management Center,Quzhou,324005)

机构地区：[1]衢州学院化学与材料工程学院,衢州324000 [2]衢州市农业林业科学研究院,衢州324000 [3]衢州市柯城区林业技术推广中心,衢州324000 [4]衢州乌溪江国家湿地公园管理中心,衢州324005

出　　处：《分子植物育种》2024年第10期3247-3252,共6页Molecular Plant Breeding

基　　金：中央财政林业科技推广示范资金项目([2020]TS10)资助。

摘　　要：本研究通过应用ISSR分子标记技术,对浙江省衢州市收集和引进的12份樱花种质的遗传多样性和亲缘关系进行了深入分析。结果表明,这些樱花种质表现出较高的遗传多样性,总多态位点百分率为76.38%,总Nei基因多样性为0.372 0,总Shannon信息指数为0.244 7。此外,通过亲缘关系分析,不同樱花种质之间的遗传相似度介于0.622 0~0.960 6之间,遗传距离在0.031 4~0.405 0之间。UPGMA聚类分析将12份樱花种质分为2个或4个主要类群,显示它们具有多样的亲缘关系。本研究的成果不仅丰富了对樱花种质资源的了解,还为其遗传多样性的保护和利用提供了宝贵的数据,为未来的樱花育种和品种改良提供了有力的支持。此外,这项研究也为其他植物遗传多样性研究提供了借鉴和参考,强调了ISSR分子标记技术在解决种质资源相关问题上的重要性。In this study,the genetic diversity and relationships of 12 Cerasus spp.germplasms collected and introduced in Quzhou,Zhejiang Province,were extensively analyzed using the ISSR molecular marker technology.The results showed that these Cerasus spp.germplasms exhibited high genetic diversity,with a total polymorphic loci percentage of 76.38%,a total Nei's gene diversity of 0.3720,and a total Shannon's information index of 0.2447.Furthermore,genetic similarity among different Cerasus spp.germplasms ranged from 0.6220 to 0.9606,with genetic distances ranging from 0.0314 to 0.4050.UPGMA cluster analysis divided the 12 Cerasus spp.germplasms into 2 or 4 major clusters,demonstrating their diverse genetic relationships.The findings of this study not only enriched our understanding of Cerasus spp.germplasms resources but also provided valuable data for the conservation and utilization of their genetic diversity.This research offers strong support for future Cerasus spp.breeding and varietal improvement.Additionally,it serves as a reference for other studies on plant genetic diversity and emphasizes the significance of the ISSR molecular marker technology in addressing germplasm-related issues.

关 键 词：樱花 种质资源 亲缘关系 遗传多样性 ISSR标记 

分 类 号：S685.99[农业科学—观赏园艺]