油梨萎蔫综合征植株根区土壤微生物组成及代谢通路分析  

Analysis of Soil Microbial Composition and Metabolic Pathways of the Avocado Wilt Complex Plants

作　　者：叶蓓蕾 张叶 刘乐 林针懿 邬静 凌鹏 YeBeilei;Zhang Ye;Liu Le;Lin Zhenyi;Wu Jing;Ling Peng(College of Horticulture,Hainan University,Haikou,570228;College of Forestry,Hainan University,Haikou,570228)

机构地区：[1]海南大学园艺学院,海口570228 [2]海南大学林学院,海口570228

出　　处：《分子植物育种》2024年第10期3355-3364,共10页Molecular Plant Breeding

基　　金：海南省重点研发计划项目(S100007118001012);海南省农业厅项目(2019-732-2)共同资助。

摘　　要：为对油梨萎蔫综合征植株根区土壤中的微生物情况做充分了解,本研究收集热带地区3个油梨种植园内萎蔫综合征植株和无症状植株根区土壤,每组3个重复共18个样品,基于宏基因组进行测序分析及其与土壤理化性质相关性分析。研究结果表明:(1)油梨萎蔫综合征植株根区土壤微生物多样性高于无症状植株根区土壤;(2)Rhodopseudomonas palustris(沼泽红假单胞菌)在萎蔫综合征植株土壤中的丰度相对于无症状植株土壤显著降低了47%,Pseudomonas unclassified(假单胞菌属)仅存在于油梨萎蔫综合征植株根区土壤中;(3)有症状植株根区土壤中速效磷含量显著高于无症状植株(P=0.019),两组间pH、有机质、速效钾和碱解氮含量无显著差异。测定的土壤理化性质分别与多个根区土壤微生物之间呈显著相关;(4)磷酸戊糖途径、倍半萜类和三萜类生物合成、糠醛降解等通路显著富集在无症状油梨植株根区土壤中,异喹啉生物碱合成途径、II型聚酮体的生物合成、铁载体非核糖体肽的生物合成等途径在有症状植株根区土壤中富集。油梨萎蔫综合征(avocado wilt complex,AWC)是全世界油梨产业中最重要的病害之一,严重制约了油梨产业的发展,土壤微生物与植物根系密切互作,与植物健康息息相关,该结果为探究油梨萎蔫综合征的致病生物和非生物因素及油梨的有效种植提供了理论基础和依据。To further study the microbial composition in the soil of root zone of avocado wilt complex plants,we collected 18 soil samples from the root zone of symptomatic plants and asymptomatic plants from three avocado plantations in tropical areas,with 3 replicates in each group,metagenomic sequencing was performed based on NovaSeq 6000 sequencing platform and the correlation analysis was performed with soil physic-chemical properties.The results showed that:(1)The soil microbial diversity in the root zone ofavocado wilt complex plants was higher than that ofasymptomatic plants.(2)The abundance of Rhodopseudomonas palustris in the soil of symptomatic plants was significantly lower than that in asymptomatic plants by 47%,which was beneficial for avocado to maintain normal growth.Pseudomonas unclassified was only presented in the soil of symptomatic plants.(3)The content of available phosphorus in the soil of the root zone of symptomatic plants was significantly higher than that of asymptomatic plants(P-0.019),and there were no significant differences in pH,soil organic matter,available potassium and alkali nitrogen content between the two groups.The measured soil physicochemical properties were significantly correlated with multiple soil microbes in root zones.(4)The metabolic pathways such as isoquinoline alkaloid biosynthesis and type II polyketide biosynthesis were shown to associated with the occurrence of avocado wilt complex;on the other hand,pentose phosphate pathway,sesquiterpene and triterpene biosynthesis,furfural degradation and other metabolisms were negatively associated with avocado wilt complex.Avocado wilt complex(AWC)is one of the most important diseases in the worldwide avocado industry,which seriously restricts the development of this industry.The interactions between soil microbes and plant roots are directly related to plant health,this study can provide a theoretical basis for exploring the biotic and abiotic factors correlated with avocado wilt complex and can provide the effective guide line for

关 键 词：油梨 萎蔫综合征 土壤微生物 宏基因组 

分 类 号：S667.9[农业科学—果树学]