光照胁迫对滇黄精多糖代谢酶活性的影响  

Effects of Light Stress on the Metabolic Enzyme Activity of Polygonatum kingianum Coll.et Hemsl.

作　　者：徐哲 王进 郭兰萍[3] 朱新焰[2] 张磊 董家红 季鹏章 Xu Zhe;Wang Jin;Gou Lanping;Zhu Xinyan;Zhang Lei;Dong Jiahong;Ji Pengzhang(Yunnan Key Laboratory of Southern Medicinal Resource,School of Chinese Materia Medica,Yunnan University of Chinese Medicine,Kunming,650500;Institute of Medicine Plants,Yunnan Academy of Agricultural Sciences,Kunming,650200;State Key Laboratory of Daodi Herbs,National Resource Center for Chinese Material Medica,China Academy of Chinese Medical Sciences,Beijing,100700)

机构地区：[1]云南中医药大学中药学院,暨云南省南药可持续利用重点实验室,昆明650500 [2]云南省农业科学院药用植物研究所,昆明650200 [3]中国中医科学院中药资源中心,道地药材国家重点实验室培养基地,北京100700

出　　处：《分子植物育种》2024年第10期3374-3381,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(No.31760347);云南省创新人才培养项目(No.2018HB085);云南中医药大学高层次人才科学研究项目(No.30270104868);云南省科技厅-云南中医药大学中医联合专项重点项目(No.202001AZ070001-010)共同资助。

摘　　要：为分析光照胁迫对滇黄精根茎多糖含量及代谢酶相关活性的影响,本研究以滇黄精为试材,利用遮阳网形成不同的光照梯度种植滇黄精。采用分光光度法测定滇黄精根茎多糖含量和蔗糖代谢相关酶(蔗糖合成酶SS,蔗糖磷酸合成酶SPS,蔗糖中性转化酶NI,蔗糖酸性转化酶AI,尿苷二磷酸葡萄糖焦磷酸化酶UGP)活性。结果表明,不同的光照胁迫对蔗糖代谢酶活性的影响不同,其中在20%透光率光照条件NI和AI活性最高,100%透光率强光照下SS、SPS和UGP活性最高。随着光照强度的增加,SS、SPS与UGP活性升高,NI和AI活性降低。通过光照强度与5种酶活性的相关性分析发现,光照强度与SS活性呈正相关。本研究表明光照能影响蔗糖代谢酶的活性,适度的光照强度有利于滇黄精根茎多糖的积累,合理调控光照强度对滇黄精多糖的积累和提高滇黄精药材的质量有指导意义。本研究可为滇黄精拟境栽培和仿野生栽培提供了基础数据和理论依据。To study the effect of light stress on the polysaccharide content and metabolic enzyme activities in the root of Polygonatum kingianum Coll.et Hemsl.,different light condition were setted for P.kingianum growth using a shading net.The polysaccharide content of rhizome and the activity of sucrose metabolism related enzymes[sucrose synthase(SS),sucrose phosphate synthase(SPS),sucrose neutral invertase(NI),sucrose acid invertase(AI)and uridine diphosphate glucose pyrophosphorylase(UGP)]were determined by spectrophotometric method.The results showed that the activity of sucrose metabolizing enzymes varied under different light stress.The NI and AI activities were the highest under 20%transmittance illumination and the SS,SPS and UGP activities were the highest under 100%transmittance illumination.With the increase of light intensity,the activities of SS,SPS and UGP increased,and the activities of NI and AI decreased.The correlation analysis between light intensity and five enzyme activities indicated that positively correlated was found between light intensity and SS activity.This study showed that light can affect the activity of sucrose metabolizing enzyme,and moderate light intensity is beneficial to the accumulation of rhizome.The reasonable regulation of light intensity has significant instruction to the accumulation of polysaccharides and the improvement of the quality of medicinal materials.The results provide a basic data and theoretical basis for the cultivation of P.kingianum and the cultivation of imitation field.

关 键 词：光照胁迫 滇黄精 多糖 蔗糖代谢酶 

分 类 号：S567.239[农业科学—中草药栽培]