病毒性出血性败血症病毒(VHSV)可视化核酸试纸条检测方法的建立  

Establishment of a Visual Nucleic Acid Strip Test for Viral Haemorrhagic Septicaemia Virus

作　　者：殷培军 金莹 唐静 岳志芹 王宫璞 房保海 YIN Pei-Jun;JIN Ying;TANG Jing;YUE Zhi-Qin;WANG Gong-Pu;FANG Bao-Hai(Technology Center of Qingdao Customs District,Qingdao 266109)

机构地区：[1]青岛海关技术中心,青岛266109

出　　处：《中国口岸科学技术》2024年第4期56-60,共5页China Port Science and Technology

基　　金：国家重点研发计划(2021YFF0602803)。

摘　　要：本研究通过在病毒性出血性败血症病毒(Viral Haemorrhagic Septicaemia Virus,VHSV)的保守区5’端设计一对保守引物,在上游和下游引物的5’端分别修饰生物素(biotin)和异硫氰酸荧光素(FITC),成功建立了VHSV快速、可视化核酸试纸条检测方法。该方法具有高灵敏度,能够检测到33拷贝/μL的VHSV,比凝胶成像检测方法提高了10倍的灵敏度。实验结果证实该方法操作程序简便、反应迅速且灵敏,具备良好的特异性,可满足现场一线对于VHSV快速检测的需求,为流行病学调查及预防控制提供了新的方法学基础。A rapid and visual nucleic acid test strip assay for Viral Haemorrhagic Septicaemia Virus(VHSV)was successfully established in this study.This was achieved by designing a pair of conserved primers at the 5'end of the conserved region of VHSV and modifying biotin and fluorescein isothiocyanate(FITC)at the 5'end of the upstream and downstream primers,respectively.The method has high sensitivity and is able to detect VHSV at 33 copies/μL,which is a 10-fold increase in sensitivity over the gel imaging detection method.The experimental results confirmed that the method had simple operating procedures,rapid response,sensitivity,and good specificity,which could meet the needs of the field for rapid detection of VHSV,and provided a new methodological basis for epidemiological investigation and prevention and control.

关 键 词：病毒性出血性败血症病毒(VHSV) 核酸试纸条 可视化检测 

分 类 号：S941.4[农业科学—水产养殖]