安徽省2023年Ⅰ型疫苗衍生脊髓灰质炎病毒鉴定及其传播风险评估  

Identification and transmission risk assessment of type I vaccine-derived poliovirus in Anhui Province in 2023

作　　者：何佳丽 王斌冰[1] 汪馨 苏颖[1] 程鹏博 董燕 宋菲扬 程晓东 HE Jiali;WANG Binbing;WANG Xin;SU Ying;CHENG Pengbo;DONG Yan;SONG Feiyang;CHENG Xiaodong(Anhui Provincial Center for Disease Control and Prevention,Hefei 230601,Anhui,China)

机构地区：[1]安徽省疾病预防控制中心,安徽合肥230601

出　　处：《安徽预防医学杂志》2024年第1期43-46,78,共5页Anhui Journal of Preventive Medicine

摘　　要：目的 分析2023年安徽省从1例非急性弛缓性麻痹(AFP)病例中分离到的2株Ⅰ型疫苗衍生脊髓灰质炎病毒(VDPV)的基因型别及特征,探讨其对安徽省维持无脊髓灰质炎状态的影响。方法 从2023年安徽省报告的1例非AFP病例双份粪便标本中分离脊髓灰质炎病毒(PV),用实时荧光定量RT-PCR方法对其进行型内鉴定,通过中和实验获得单型毒株,提取病毒RNA,进行RT-PCR扩增,对VP1区进行核苷酸序列测定和分析。结果 从1例非AFP病例粪便标本中分离出2株Ⅰ+Ⅲ混合型PV,其中Ⅰ型(2023MYKJH 01-1和2023MYKJH 01-2)分别有13和12个核苷酸变异,变异率分别为1.4%和1.3%,鉴定为Ⅰ型VDPV,与近年来我国其他省份监测到的Ⅰ型VDPV处于不同分支,Ⅲ型为疫苗相似株。后续采集的粪便标本未分离到Ⅰ型VDPV。结论 2023年安徽省检出两株Ⅰ型VDPV的非AFP病例未持续排毒,发生Ⅰ型VDPV循环的风险较低。Objective To analyze the genotypes and characteristics of two vaccine-derived poliovirus(VDPV)strains isola-ted from a non-acute flaccid paralysis(non-AFP)case in Anhui Province in 2023,and to explore the influence of this case on maintaining polio-free status in Anhui province.Methods Poliovirus(PV)was isolated from two samples of a non-AFP case in Anhui Province in 2023.The PV was identified by real time fluorescent quantitative RT-PCR.The single type strain was obtained by neutralization experiment,the viral RNA was extracted and amplified by RT-PCR,and the nucleotide se-quence of VP1 region was determined and analyzed.Results Two mixed type I+III PV strains were isolated from the doub-le fecal samples of the non-AFP case.Type I(2023MYKJH 01-1 and 2023MYKJH 01-2)had 13 and 12 nucleotide vari-ants,with variation rates of 1.4%and 1.3%,respectively,they were identified as type I VDPV and in a different branch from the type I VDPV detected in other province in China in recent years.Type III were vaccine-like strains.Type I VDPV was not isolated from subsequent stool samples.Conclusion One non-AFP case of two type I VDPV strains detected in Anhui Province in 2023 did not undergo continuous detoxification,resulting in a lower risk of developing type I VDPV circu-lation.

关 键 词：疫苗衍生脊髓灰质炎病毒 型内鉴定 序列分析 

分 类 号：R512.4[医药卫生—内科学]