可溶性肿瘤坏死因子样凋亡弱诱导因子在斑秃病人血清及皮损中的表达及临床意义  

Expression and clinical significance of sTWEAK in the serum and skin lesions of patients with alopecia areata

作　　者：张茜[1] 孙菲[1] 王刚[1] 郑燕 ZHANG Qian;SUN Fei;WANG Gang;ZHENG Yan(Clinical Laboratory Center,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院临床检验中心,北京100050

出　　处：《安徽医药》2024年第6期1221-1225,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的检测可溶性肿瘤坏死因子样凋亡弱诱导因子(sTWEAK)在斑秃病人血清及皮损中的表达水平,并探讨其在斑秃发病过程中的作用及临床意义。方法以2017年10月至2018年11月在首都医科大学附属北京友谊医院确诊并治疗的斑秃病人70例为斑秃组,依据病情分为轻症组(44例)、重症组(26例)。同期该院因色素痣或皮肤良性肿瘤进行头皮手术的人群65例作为对照组。比较各组sTWEAK及相关细胞因子白细胞介素(IL)-4、IL-12、γ干扰素(IFN-γ)蛋白及mRNA表达水平;采用Pearson法分析两指标间相关性。多元线性回归分析各指标与sTWEAK或脱发严重度工具(SALT)评分的关系。结果斑秃组血清及皮损中sTWEAK表达水平[(196.73±32.48)ng/L,(2.35±0.62)ng/L]高于对照组[(121.75±19.62)ng/L,(1.49±0.45)ng/L](P<0.05)。斑秃组血清及皮损中细胞因子IL-12、IFN-γ表达水平高于对照组(P<0.05),IL-4表达水平比较差异无统计学意义(P>0.05)。斑秃病人血清及皮损中sTWEAK表达水平与IL-12及IFN-γ呈正相关,与IL-4表达水平无明显相关性。轻症组血清及皮损中sTWEAK[(173.25±26.84)ng/L,(2.04±0.57)ng/L]、IL-12及IFN-γ表达水平低于重症组[(236.47±42.02)ng/L,(2.87±0.70)ng/L](P<0.05);轻症组、重症组血清及皮损中IL-4表达水平差异无统计学意义(P>0.05);斑秃病人血清、皮损处sTWEAK、IL-12、IFN-γ与SALT评分均呈正相关(P<0.05)。多元线性回归分析显示,IL-12、IFN-γ与血清和皮损处sTWEAK呈显著正相关;血清及皮损处sTWEAK、IL-12、IFN-γ与SALT评分存在正相关性(P<0.05)。结论斑秃病人血清及皮损中sTWEAK表达水平增加,与IL-12及IFN-γ呈正相关,sTWEAK、IL-12及IFN-γ均与斑秃病情严重程度密切相关,sTWEAK可能参与斑秃炎症反应过程。Objective To detect the expression level of soluble tumor necrosis factor like weak inducer of apoptosis(sTWEAK)in the serum and skin lesions of alopecia areata patients,and to explore its role and clinical significance in the pathogenesis of alopecia areata.Methods A total of 70 patients with alopecia areata who were diagnosed and treated at the Department of Dermatology,Beijing Friendship Hospital,Capital Medical University,between October 2017 and November 2018 were selected for the alopecia areata group;these patients were divided into a mild group(44 cases)and a severe group(26 cases)according to their condition.During the same period,65 patients who underwent scalp surgery for pigmented nevi or benign skin tumors in the surgery department of the hospital served as the control group.The protein and mRNA expression levels of sTWEAK and the related cytokines interleukin(IL)-4,IL-12 and interferon-γ(IFN-γ)were compared among the groups.Pearson's method was used to analyze the correlation between the two indices.Multivariate linear regression was used to analyze the relationship between each index and the sTWEAK score or severity of alopecia tool(SALT)score.Results The levels of sTWEAK expression in serum and skin lesions in the alopecia areata group[(196.73±32.48)ng/L,(2.35±0.62)ng/L]were greater than those in the control group[(121.75±19.62)ng/L,(1.49±0.45)ng/L](P<0.05).The expression levels of the cytokines IL-12 and IFN-γin the serum and skin lesions of the alopecia areata group were greater than those in the control group(P<0.05),and there was no significant difference in the expression level of IL-4 between the two groups(P>0.05).The expression level of sTWEAK in the serum and skin lesions of patients with alopecia areata was positively correlated with that of IL-12 and IFN-γand was not significantly correlated with the IL-4 expression level(P>0.05).The expression levels of sTWEAK[(173.25±26.84)ng/L and(2.04±0.57)ng/L],IL-12 and IFN-γin the serum and skin lesions in the mild group were lower tha

关 键 词：斑秃 可溶性肿瘤坏死因子样凋亡弱诱导因子 血清 皮损 细胞因子 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]