Dendrimer-induced synthesis of porous organosilica capsules for enzyme encapsulation  

作　　者：Ziyi Chu Boyu Zhang Zhenhua Wu Jiaxu Zhang Yiran Cheng Xueying Wang Jiafu Shi Zhongyi Jiang 

机构地区：[1]School of Environmental Science&Engineering,Tianjin University,Tianjin 300072,China [2]Collaborative Innovation Center of Chemical Science and Engineering,Tianjin 300072,China [3]State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China [4]Key Laboratory for Green Chemical Technology of Ministry of Education,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China

出　　处：《Frontiers of Chemical Science and Engineering》2024年第4期41-48,共8页化学科学与工程前沿（英文版）

基　　金：supported by the National Key Research and Development Program of China(Grant No.2021YFC210-2300);the National Key Research and Development Program of China(Grant No.2022YFC2105902);Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(Grant No.TSBICIP-KJGG-003);Open Funding Project of the State Key Laboratory of Biochemical Engineering of China(Grant No.2020KF-06);Haihe Laboratory of Sustainable Chemical Transformations.

摘　　要：Organic matter-induced mineralization is a green and versatile method for synthesizing hybrid nanostructured materials,where the material properties are mainly influenced by the species of natural biomolecules,linear synthetic polymer,or small molecules,limiting their diversity.Herein,we adopted dendrimer poly(amidoamine)(PAMAM)as the inducer to synthesize organosilica-PAMAM network(OSPN)capsules for mannose isomerase(MIase)encapsulation based on a hard-templating method.The structure of OSPN capsules can be precisely regulated by adjusting the molecular weight and concentration of PAMAM,thereby demonstrating a substantial impact on the kinetic behavior of the MIase@OSPN system.The MIase@OSPN system was used for catalytic production of mannose from Dfructose.A mannose yield of 22.24% was obtained,which is higher than that of MIase in organosilica network capsules and similar to that of the free enzyme.The overall catalytic efficiency(kcat/Km)of the MIase@OSPN system for the substrate D-fructose was up to 0.556 s^(-1)·mmol^(-1)·L.Meanwhile,the MIase@OSPN system showed excellent stability and recyclability,maintaining more than 50% of the yield even after 12 cycles.

关 键 词：enzyme immobilization enzyme catalysis organosilica networks CAPSULES sugar biosynthesis 

分 类 号：O64[理学—物理化学]