锥形光纤SERS探针的批量制备和定量检测  

Batch Preparation and Quantitative Detection of Tapered SERS Fiber Probes

作　　者：秦琰琰 黄瑞冬 刘孝兵 钱诚 薛驷明 毛庆和 Qin Yanyan;Huang Ruidong;Liu Xiaobing;Qian Cheng;Xue Siming;Mao Qinghe(Anhui Provincial Key Laboratory of Photonics Devices and Materials,Anhui Institute of Optical and Fine Mechanics,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,Anhui,China;University of Science and Technology of China,Hefei 230026,Anhui,China;Institutes of Physical Science and Information Technology,Anhui University,Hefei 230039,Anhui,China)

机构地区：[1]中国科学院合肥物质科学研究院安徽光学精密机械研究所安徽省光子器件与材料重点实验室,安徽合肥230031 [2]中国科学技术大学,安徽合肥230026 [3]安徽大学物质科学与信息技术研究院,安徽合肥230039

出　　处：《中国激光》2024年第5期251-259,共9页Chinese Journal of Lasers

基　　金：安徽省科技重大专项(201903a07020021);科技创新2030重大项目。

摘　　要：研究了批量制备的锥形光纤表面增强拉曼散射(SERS)探针的定量检测性能。在统一的制备条件下,基于静电吸附自组装方法批量制备的同一批次锥形光纤探针具有良好的互换性。基于相同检测条件,在同一福美双样品浓度下测得SERS光谱幅度的相对标准偏差(RSD)可达8%以下。不同批次的光纤探针存在互换性退化问题,难以满足实际定量检测应用对光纤探针数目的要求。为了解决该问题,提出并演示了一种将不同批次光纤探针的光谱数据同化至同批次光纤探针测量结果的同化方法。通过对同化后的大样本光谱数据进行统计平均和数据拟合,获得了福美双样品在2×10^(-8)~10^(-6)mol/L浓度范围内的SERS定量关系曲线,福美双加标样品的测试回收率可达90%~110%。该研究结果对于实际SERS定量检测具有参考意义。Objective Surface-enhanced Raman scattering(SERS)spectroscopy has significant applications in various fields such as food safety,environmental monitoring,and life sciences.In recent years,there has been growing interest in the quantitative detection of substance concentrations using SERS spectroscopy.SERS fiber probes,which offer outstanding practical value,enable in situ and onsite detection of substances in complex environments,making them highly suitable for practical quantitative measurements.However,because the reuse of fiber probes is challenging owing to contamination by the substances being measured,it is essential to prepare a large number of fiber probes that exhibit good interchangeability in batches.This allows the establishment of a statistical quantitative relationship between the spectral amplitude and substance concentration through large-sample spectral detection.This statistical quantitative relationship can thereafter be used to determine the concentrations of unknown samples.However,the interchangeability differences of fiber probes prepared from similar and different batches and the elimination or compensation for interchangeability degradation during batch preparation for quantitative detection have not yet been studied.In this study,we employ an electrostatic adsorption self-assembly method to prepare tapered SERS fiber probes.By assimilating,statistically averaging,and fitting large-sample spectral data measured from different batches of fiber probes,we obtain high-precision quantitative curves and achieve quantitative detection of thiram samples.Methods A batch of bare tapered fibers is fixed onto a specially designed disc.Subsequently,a monolayer of uniformly distributed gold nanospheres is grown on the surface of these fibers at the same density under the optimized electrostatic adsorption self-assembly conditions.Subsequently,a batch of tapered SERS fiber probes with excellent interchangeability is obtained.Tapered SERS fiber probes are prepared by repeating the same preparation proces

关 键 词：光纤光学 表面增强拉曼散射 锥形光纤探针 批量制备 定量检测 静电吸附自组装法 福美双 

分 类 号：O433.4[机械工程—光学工程]