三维拉曼显微成像技术研究进展(特邀)  

Research Progress of Three-Dimensional Raman Microscopic Imaging(Invited)

作　　者：冯巩 行霆燕 王楠 曾琦 谢晖 陈雪利 Feng Gong;Xing Tingyan;Wang Nan;Zeng Qi;Xie Hui;Chen Xueli(Xi'an Key Laboratory of Intelligent Sensing and Regulation of Trans-Scale Life Information,School of Life Science and Technology,Xidian University,Xi'an 710126,Shaanci,China;Innovation Center for Advanced Medical Imaging and Intelligent Medicine,Guangzhou Institute of Technology,Xidian University,Guangzhou 510555,Guangdong,China)

机构地区：[1]西安电子科技大学生命科学技术学院西安市跨尺度生命信息智能感知与调控重点实验室,陕西西安710126 [2]西安电子科技大学广州研究院先进医学影像与智慧医疗创新中心,广东广州510555

出　　处：《激光与光电子学进展》2024年第6期175-187,共13页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(62275210,62007026);国家级青年人才项目;陕西省杰出青年科学基金(2020JC-27);陕西省青年拔尖人才项目;陕西省重点研发计划重点产业创新链项目(2021ZDLSF04-05);陕西省重点研发计划一般项目(2022GY-313)。

摘　　要：拉曼显微成像技术无需样本制备,具有无损、无创、对水溶液不敏感的优点,可在微米或纳米尺度下表征样本的生化组分及分布,成为生命科学领域重要的研究工具。随着对复杂生物样本研究的不断深入,拉曼显微成像也被期待能够实现对生物样本中的分子组成与分布的动态立体观测。首先,系统性地梳理近年来三维拉曼显微成像技术的研究进展,包括基于自发拉曼散射、相干拉曼散射、表面增强拉曼散射以及拉曼标签的不同三维成像方法的技术手段、改进策略与实验结果。然后,总结了不同成像技术在细胞生物学、发育生物学等方面的应用进展。最后展望了不同三维拉曼显微成像技术在生物医学光学显微成像技术应用中所面临的挑战和发展前景。Raman microscopic imaging has emerged as an important research tool in life sciences because it does not require sample preparation and is non-destructive,non-invasive,and insensitive to aqueous solutions.It enables the characterization of biochemical components and sample distributions at a micrometer or nanometer scale.As the research on complex biological samples increases,Raman microscopic imaging holds potential for the dynamic stereoscopic observation of molecular composition and distribution in biological samples.This paper systematically examined recent advancements in 3D Raman microscopic imaging,including technical approaches,improvement strategies,and experimental results of various 3D imaging methods based on spontaneous Raman scattering,coherent Raman scattering,surface-enhanced Raman scattering,and Raman tags.Further,the progress of the applications of different imaging techniques in cell biology and developmental biology was summarized.Finally,the challenges and development prospects of different 3D Raman microscopic imaging techniques in the application of biomedical optical microscopic imaging technology were outlined.

关 键 词：三维显微成像 拉曼显微成像 自发拉曼散射 相干拉曼散射 表面增强拉曼散射 拉曼标签 

分 类 号：O436[机械工程—光学工程]