Super-Resolution Fluorescence Microscopy for Cilia Investigation and Ciliopathy Diagnosis(Invited)  

作　　者：Liu Zhen Wu Yang 

机构地区：[1]Division of Life Science,The Hong Kong University of Science and Technology,Hong Kong,China

出　　处：《激光与光电子学进展》2024年第6期260-271,共12页Laser & Optoelectronics Progress

基　　金：supported by the General Research Fund(GRF)from the Research Grants Council of Hong Kong(16100823,26101022)to Z.L.

摘　　要：The last two decades have witnessed the invention and development of super-resolution microscopy(SRM)that breaks the diffraction limit of light and pushes the fluorescence microscopy resolution to several nanometers.While SRM is widely used in biological studies,such as resolving subdiffraction structures,molecular mapping,tracking single molecules,probing protein-protein interactions,and observing organelle dynamics,its direct application in translational medicine,such as disease diagnosis,is still preliminary.Despite their small size,cilia play a crucial role as organelles in cell signaling and motility,with defects in cilia leading to ciliopathy.Similar to other miniature organelles and macromolecular complexes,cilia are ideal for super-resolution imaging.In this review,we will 1)introduce cilia and ciliopathy,2)show how SRM extends our knowledge of cilia,and 3)focus on how SRM improves the diagnosis of motile ciliopathies.

关 键 词：DIAGNOSIS LIMIT SPITE 

分 类 号：TH742[机械工程—光学工程]