植物甾醇中豆甾醇分离提纯的工艺研究  

Study on Separation and Purification of Stigmasterol From Phy tosterol

作　　者：关国华 武文华 蒋一鸣 周学晋 徐逍然 宋莲芳 胡志清 梁广震 史金柱 GUAN Guohua;WU Wenhua;JIANG Yiming;ZHOU Xuejin;XV Xiaoran;SONG Lianfang;HU Zhiqing;LIANG Guangzhen;SHI Jinzhu(COFCO TECH Bioengineering(Tianjin)Co.,Ltd.,Tianjin 300457,China;COFCO Biotechnology Co.,Ltd.,Bengbu Anhui 233010,China)

机构地区：[1]中粮天科生物工程(天津)有限公司,天津300457 [2]中粮生物科技股份有限公司,安徽蚌埠233010

出　　处：《当代化工》2024年第3期722-725,共4页Contemporary Chemical Industry

摘　　要：植物甾醇是一种存在于植物中的天然活性物质,被广泛应用于维生素D、多种激素和甾族化合物的合成。目前工业生产常以大豆油脂的脱臭馏出物为原料,通过甲酯化提取混合植物甾醇。已知豆甾醇在环己酮和正戊醇中的溶解度随温度降低而下降。采用溶剂结晶法,分离提纯植物甾醇中的豆甾醇单体。结果表明:采用料液比1∶3,加热至65℃溶解后进行控温降温养晶,经过3次结晶过程豆甾醇单体含量(本文中甾醇含量均为质量分数)可达到76.89%,收率46.37%。Phytosterol is a naturally active substance that exists in plants and is widely used in the synthesis of vitamin D,various hormones,and steroid compounds.Currently,industrial production often uses the deodorized distillate of soybean oil as raw material to extract mixed phytosterols through methylation.It is known that the solubility of stigmasterol in cyclohexanone and n-pentanol decreases with the decrease of temperature.In this paper,a solvent crystallization method was used to separate and purify the monomers of stigmasterol from phytosterols.The experimental results showed that the mass fraction of stigmasterol monomer could reach 76.89%and the yield was 46.37%by using a material liquid ratio of 1:3,heating to 65℃ for dissolution,and then conducting temperature control,temperature reduction,and crystallization.

关 键 词：植物甾醇 豆甾醇 环己酮 正戊醇 提纯 

分 类 号：TQ028[化学工程]