人参交链格孢菌对氯氟醚菌唑的敏感基线和抗药性监测  

Monitoring of Alternaria alternata Fungicide Resistance to Mefentrifluconazole from Panax ginseng

作　　者：张嘉怡 冯志伟 侯万鹏 卢宝慧 栾奕[3] 高洁 ZHANG Jiayi;FENG Zhiwei;HOU Wanpeng;LU Baohui;LUAN Yi;GAO Jie(College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;Jilin Shenwang Plant Protection Science and Technology Co.Ltd.,Baishan 134300,China;Jilin Province Seed Management Station,Changchun 130033,China)

机构地区：[1]吉林农业大学植物保护学院,长春130118 [2]吉林参王植保科技有限公司,白山134300 [3]吉林省种子管理总站,长春130033

出　　处：《菌物研究》2024年第2期191-195,共5页Journal of Fungal Research

基　　金：吉林省科技发展计划项目(20210204047YY)。

摘　　要：为进一步了解人参交链格孢菌Alternaria alternata对氯氟醚菌唑的抗药性情况,并明确吉林省各地区(白山市、敦化市、通化市、吉林市、长春市)人参交链格孢菌的抗药性水平。采用菌丝生长速率法测定了人参交链格孢菌对氯氟醚菌唑的敏感性,建立其敏感基线,并根据抗药性水平分级标准评价其抗性水平。结果表明:氯氟醚菌唑对吉林省人参产区A. alternata抑菌活性较高,108株人参交链格孢菌对氯氟醚菌唑的敏感性频率分布符合正态分布,其所有敏感菌株EC50的平均值(0.975 5±1.183 5)μg/mL可作为人参交链格孢菌对氯氟醚菌唑的敏感基线。目前吉林省的人参交链孢菌对氯氟醚菌唑处于低抗药性水平,研究结果为氯氟醚菌唑对人参链格孢菌的抗药性风险评估奠定了基础。In order to clasify the drug resistance of Alternaria alternata distributing within different regions(Baishan, Dunhua, Tonghua, Jilin, and Changchun) of Jilin Province, its sensitivity to mefentrifluconazole was investigated through the determination of mycelial growth rate, and a sensitive baseline of A. alternata to mefentrifluconazole was successfully established at the present study. According to the grading standards of fungicide-resistance, furthereomre, evaluation of resistance ability(or sensitivity) was conducted. The results showed that mefentrifluconazole efficiently inhibits the activity of A. alternata isolates in vitro. The sensitivity frequence of mefentrifluconazole against mycelial growth detected from all of 108 isolates was confirmed statistically to show a normal distribution, in which the average EC_(50) value(0.975 5±1.183 5 μg/mL)could be used as a sensitive baseline of A. alternate to the mefentrifluconazole. It was found that A.alternata populations currently present in Jilin Province carried a low level of drug resistance to mefentrifluconazole. The results obtained from this study provide an important foundation for the risk assessment of A. alternata resistance to mefentrifluoconazole.

关 键 词：人参 交链格孢菌 氯氟醚菌唑 敏感基线 抗药性监测 

分 类 号：Q949.325[生物学—植物学] S432[农业科学—植物病理学]