基于AHP-熵权法结合正交试验优选银杏叶传统水提纯化工艺  

Optimization of traditional water extraction and purification process for Ginkgo folium based on AHP-entropy weight method combined with orthogonal experiment

作　　者：姜誉弘 邹恺平 JIANG Yuhong;ZOU Kaiping(Department of Pharmacy,The Affiliated Hospital of Nanjing University of Chinese Medicine(Jiangsu Province Hospital of Chinese Medicine),Nanjing 210029,China;Department of Preparation,The Affiliated Hospital of Nanjing University of Chinese Medicine(Jiangsu Province Hospital of Chinese Medicine),Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院(江苏省中医院)药学部,南京210029 [2]南京中医药大学附属医院(江苏省中医院)制剂部,南京210029

出　　处：《中国药师》2024年第5期747-755,共9页China Pharmacist

基　　金：南京药学会—常州四药医院药学科研基金资助项目(2020YX012)。

摘　　要：目的 优选银杏叶最佳传统水提工艺和纯化工艺。方法 以总银杏酸转移率、总黄酮醇苷转移率和出膏率为考察指标,采用层次分析法(AHP)-熵权法确定权重系数,在单因素试验的基础上,采用正交试验优选出银杏叶的最佳水提和纯化工艺。结果 最佳水提工艺为:银杏叶饮片装量比为1/2,加8倍水,提取2次,每次30 min;最佳纯化工艺为:水提药液减压浓缩至相对密度1.08,4℃静置48 h,静置后的上层药液2 029×g离心5 min。结论 该方法稳定可行,能最大程度地去除有害成分总银杏酸,保留有效成分总黄酮醇苷。Objective To optimize the traditional water extraction and purification process of Ginkgo folium.Methods Using the transfer rate of total ginkgolic acid,transfer rate of total flavonol glycosides,and extraction rate as evaluation indicators,the weight coefficient was determined by analytic hierarchy process(AHP)-entropy weight method.On the basis of single factor experiments,the optimal water extraction and purification process for Ginkgo folium were selected through orthogonal experiments.Results The optimum water extraction process was as follows:the ratio of ginkgo leaf pieces was 1/2,adding 8 times of water,decocting twice,and 30 min for each time.The optimum purification process was as follows:the water extract was concentrated under reduced pressure to a relative density of 1.08 and stewing at 4 °C for 48 h,and the upper layer of medicine liquid a?ter stewing was centrifugated for 5 min at 2029×g.Conclusion The method is stable and feasible,which can remove the harmful component,total ginkgolic acid to the greatest extent possible,and retain the effective component,total flavonol glycosides.

关 键 词：银杏叶 水提 纯化 总银杏酸 总黄酮醇苷 层次分析法-熵权法 

分 类 号：R284.2[医药卫生—中药学]