海岛女性早期复发性流产患者核因子κB 血红素氧合酶-1 单核细胞趋化蛋白-1表达特点及与妊娠结局的相关性  

Expression characteristics of NF-KB, HO-1, MCP-1 and their correlation with pregnancy outcome in island women with early recurrent abortion

作　　者：罗慧琴 邬贤凤 吴佳洁 LUO Hui-qin;WU Xian-feng;WU Jia-jie(Department of Gynecology,Zhoushan Hospital,Zhoushan,Zhejiang 316000,China)

机构地区：[1]舟山医院妇科,浙江舟山316000

出　　处：《中国妇幼保健》2024年第9期1634-1637,共4页Maternal and Child Health Care of China

基　　金：浙江省舟山市科技计划项目(2019C31099)。

摘　　要：目的 探究海岛女性早期复发性流产患者核因子κB(NF-κB)、血红素氧合酶-1(HO-1)、单核细胞趋化蛋白-1(MCP-1)表达特点及与妊娠结局的相关性。方法 选取2019年5月—2021年5月舟山医院妇科收治的30例海岛早期复发性流产患者为观察组受试者,另选择同期正常早孕要求人工流产终止妊娠的女性30例为对照组受试者。分别采用PCR、Western及免疫组化方法检测两组研究对象绒毛和蜕膜组织中HO-1、MCP-1及NF-κB的含量变化情况。结果 PCR检测结果显示,观察组受试者的NF-κB、HO-1及MCP-1指标水平[(0.98±0.11)、(1.94±0.08)及(90.84±4.98)]均高于对照组受试者[(0.69±0.05)、(1.45±0.13)及(62.24±3.64)],差异均有统计学意义(t=16.346、13.465及32.165,均P<0.05)。Western检测结果显示,观察组受试者的NF-κB、HO-1及MCP-1指标水平[(0.94±0.14)、(1.54±0.26)及(91.58±4.67)]均高于对照组受试者[(0.68±0.07)、(1.09±0.02)及(61.46±3.66)],差异均有统计学意义(t=10.485、21.596及36.459,均P<0.05)。免疫组化方法检测结果显示,观察组受试者的NF-κB、HO-1及MCP-1指标水平[(0.96±0.19)、(1.76±0.15)及(98.49±4.90)]均高于对照组受试者[(0.65±0.05)、(1.12±0.04)及(60.12±3.31)],差异均有统计学意义(t=6.545、19.255及29.782,均P<0.05)。多因素logistic回归分析显示,NF-κB、HO-1及MCP-1是早期复发性流产患者妊娠结局的独立危险因素(均P<0.05)。Pearson分析结果显示,早期复发性流产患者NF-κB、HO-1及MCP-1与妊娠结局均呈正相关关系(r=4.685、4.763及5.342,均P<0.05)。结论 早期的复发性流产患者绒毛和蜕膜组织中的NF-κB、MCP-1及HO-1表达水平较高。NF-κB、MCP-1及HO-1指标异常是引发流产的危险因素之一。对早期复发性流产的患者,检测NF-κB、MCP-1及HO-1表达水平,有利于降低再次不良妊娠结局发生率,早期复发性流产患者胎盘中的NF-κB、MCP-1及HO-1表达与妊娠结局具有密切联系。Objective To investigate the expression characteristics of NF-κB,HO-1,and MCP-1 in women with early recurrent abortion on islands and their correlation with pregnancy outcomes.Methods Thirty patients with early recurrent abortion admitted to the Department of Gynecology,Zhoushan Hospital from May 2019 to May 2021 were selected as the observation group,and 30 healthy women who requested artificial abortion termination of early pregnancy during the same period were selected as the control group.The changes in the expression of HO-1,MCP-1,and NF-κB in the villus and decidua tissues of the two groups were detected by PCR,Western blot,and immunohistochemistry.Results PCR detection showed that the expression levels of NF-κB,HO-1,and MCP-1 in the observation group[(0.98±0.11),(1.94±0.08),and(90.84±4.98)]were higher than those in the control group[(0.69±0.05),(1.45±0.13),and(62.24±3.64)],with statistically significant differences(t=16.346,13.465,and 32.165,all P<0.05).Western blot detection showed that the expression levels of NF-κB,HO-1,and MCP-1 in the observation group[(0.94±0.14),(1.54±0.26),and(91.58±4.67)]were higher than those in the control group[(0.68±0.07),(1.09±0.02),and(61.46±3.66)],with statistically significant differences(t=10.485,21.596,and 36.459,all P<0.05).Immunohistochemical staining showed that the expression levels of NF-κB,HO-1,and MCP-1 in the observation group[(0.96±0.19),(1.76±0.15),and(98.49±4.90)]were higher than those in the control group[(0.65±0.05),(1.12±0.04),and(60.12±3.31)],with statistically significant differences(t=6.545,19.255,and 29.782,all P<0.05).Multivariate logistic regression analysis showed that NF-κB,HO-1,and MCP-1 were independent risk factors for pregnancy outcomes in patients with early recurrent abortion(all P<0.05).Pearson analysis showed a positive correlation between NF-κB,HO-1,and MCP-1 expression levels and pregnancy outcomes in patients with early recurrent abortion(r=4.685,4.763,and 5.342,all P<0.05).Conclusion The expression levels of NF-�

关 键 词：早期复发性流产 核因子ΚB 血红素氧合酶-1 单核细胞趋化蛋白-1 妊娠结局 相关性 

分 类 号：R711[医药卫生—妇产科学]