德宏傣族景颇族自治州2018―2019年新报告人类免疫缺陷病毒感染者HIV B和CRF01_AE以及C亚型分子传播网络特征分析  

Molecular transmission network characteristics of HIV-1 subtypes B,CRF01_AE and C in newly reported human immunodeficiency virus infections in Dehong Dai and Jingpo Autonomous Prefecture during 2018-2019

作　　者：段星 王雪琪 王继宝 王译葵 杨锦 周素娟 杨涛 曹艳芬 杨跃诚 唐仁海 叶润华 丁盈盈 何纳 段松 DUAN Xing;WANG Xueqi;WANG Jibao;WANG Yikui;YANG Jin;ZHOU Sujuan;YANG Tao;CAO Yanfen;YANG Yuecheng;TANG Renhai;YE Runhua;DING Yingying;HE Na;DUAN Song(不详;Department of STD/AIDS Prevention and Control,Dehong Prefecture Center for Disease Control and Prevention,Mangshi,Yunnan 678400,China)

机构地区：[1]德宏傣族景颇族自治州疾病预防控制中心艾滋病性病防制科,云南芒市678400 [2]复旦大学公共卫生学院流行病学教研室公共卫生安全教育部重点,上海200032

出　　处：《中国病毒病杂志》2024年第1期25-30,共6页Chinese Journal of Viral Diseases

基　　金：国家科技重大专项(2018ZX10721102-004,2018ZX10715006-001)。

摘　　要：目的分析德宏傣族景颇族自治州(德宏州)2018―2019年新报告HIV感染者中B、CRF01_AE、C亚型的分子传播网络特征,为实现艾滋病精准防控和干预提供科学依据。方法通过全国艾滋病综合防治数据信息系统,收集2018―2019年德宏州新报告人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者的相关信息,分析新报告HIV感染者的人口学特征。选择HIV感染者外周血样本中血浆量≥200μl的样本进行HIV pol基因扩增、测序、拼接、比对和分型,运用Mega 7.0、Gephi 0.10.1软件用于构建分子传播网络。结果2018―2019年德宏州新报告HIV感染者中,中国籍HIV感染者300例(占33.3%),缅甸籍HIV感染者600例(占66.7%),他们在年龄、婚姻、民族、文化程度、感染途径、基因亚型、报告地、接触史上的分布差异均有统计学意义。单因素logistic回归分析结果显示缅甸籍HIV感染者通过注射毒品方式感染HIV的是中国籍HIV感染者的6.855(OR:6.855,95%CI:4.075~11.531)倍。此外,HIV B、CRF01_AE、C亚型的分子网络分析结果显示,在B亚型的分子传播网络中出现了一个独立于主要传播簇(大簇)的HIV活跃传播簇,且此簇有不断扩大的趋势。该簇中8例HIV感染者均为35岁以下缅甸籍男性,集中在陇川、盈江两县,并且其性别、年龄、文化程度等具有同质性。结论德宏州境内HIV-1感染者中B亚型缅甸籍感染者形成了独立的活跃传播簇,有明显地域和人群聚集特征,并呈不断扩大趋势。应密切关注德宏州陇川、盈江两县HIV活跃传播网络中的感染者,做到精准防控,降低艾滋病的传播流行。Objective To analyze the characteristics of molecular transmission network of HIV-1 subtypes B,CRF01_AE and C among newly reported HIV-infected patients in Dehong Dai and Jingpo Autonomous Prefecture(Dehong prefecture)from 2018 to 2019,and provide scientific support for precise HIV prevention and control.Methods Data on newly reported HIV-infected patients in Dehong prefecture from 2018 to 2019 were obtained from the national comprehensive HIV/AIDS prevention and control information system and the demographic characteristics of newly reported HIV infections were analyzed.Peripheral blood samples containing more than 200μl plasma from HIV-infected patients were selected for amplification,sequencing,splicing,alignment and typing of the HIV pol gene.Mega 7.0 and Gephi 0.10.1 software were used for constructing molecular transmission network.Results There were 300(33.3%)Chinese and 600(66.7%)Burmese HIV infections in newly reported HIVinfected patients in Dehong prefecture from 2018 to 2019.There were statistically significant difference in distribution of age,marriage、nation,education level,infection route,gene subtype,reporting location,contact history between Chinese and Burmese patients.Univariate logistic regression analysis showed that Burmese patients were 6.855 times more likely than Chinese patients to contract HIV by injecting drug use(OR=6.855,95%CI:4.075—11.531).In addition,according to analysis of the molecular transmission network of HIV-1 subtypes B,CRF01_AE and C,the molecular transmission network of HIV-1 subtype B had an active HIV transmission cluster that was independent of the main transmission cluster(large cluster),and this cluster tended to expand continuously.HIV infections in this cluster were all Burmese male under 35 years old,concentrated in Longchuan and Yingjiang counties,and their gender,age and education level were homogenous.Conclusions Among HIV-1 infections in Dehong prefecture,burmese infections with subtype B formed a distinct active transmission cluster,demonstrating notabl

关 键 词：人类免疫缺陷病毒 艾滋病 基因亚型 分子传播网络 HIV活跃传播簇 

分 类 号：R512.91[医药卫生—内科学]